Overview
- External ontology
- MEDIADB:202 (source link unavailable) — Medium without amino acids; fang et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007095["medium_without_amino_acids_fang_et_al"]
ing0_Glycerol["Glycerol"]
CultureMech_007095 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_Starch["Starch"]
CultureMech_007095 --> ing1_Starch
CHEBI_28017["CHEBI:28017"]
ing1_Starch -.-> CHEBI_28017
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007095 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007095 --> ing3_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing4_Sodium_sulfate["Sodium sulfate"]
CultureMech_007095 --> ing4_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing4_Sodium_sulfate -.-> CHEBI_32149
ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007095 --> ing5_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing6_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007095 --> ing6_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing6_Magnesium_sulfate -.-> CHEBI_32599
ing7_Manganese_sulfate["Manganese sulfate"]
CultureMech_007095 --> ing7_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing7_Manganese_sulfate -.-> CHEBI_86364
ing8_Ammonium_chloride["Ammonium chloride"]
CultureMech_007095 --> ing8_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing8_Ammonium_chloride -.-> CHEBI_31206
ing9_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007095 --> ing9_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing9_Ferrous_sulfate -.-> CHEBI_75832
ing10_Ammonium_nitrate["Ammonium nitrate"]
CultureMech_007095 --> ing10_Ammonium_nitrate
CHEBI_63038["CHEBI:63038"]
ing10_Ammonium_nitrate -.-> CHEBI_63038
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
27.147 MILLIMOLAR |
CHEBI:17754
glycerol
|
Cross-references: KEGG:glyc |
| 2 | Starch | 154.32 MILLIMOLAR |
CHEBI:28017
starch
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.0090106 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
34.443 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
1.408 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 6 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
14.6965 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.16229 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.008965 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
18.6947 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 10 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0071929 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 11 |
Ammonium nitrate
H4N.NO3 80.043 g/mol
|
2.49837 MILLIMOLAR |
CHEBI:63038
ammonium nitrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_without_amino_acids_fang_et_al (CultureMech:007095)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.395946Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 202, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.104658Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007095 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.236110+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium without amino acids; fang et al" to "medium_without_amino_acids_fang_et_al" for consistent matching |
| 2026-06-05T07:41:30.412637+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:44.403822+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 414ce726644dea98e7dbf1a06e1b19cb0a5381ecb87a03205bdcdd1ca2a15acd |