Overview
- External ontology
- MEDIADB:206 (source link unavailable) — Deshpande medium with Met, Asp; Fang et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007099["deshpande_medium_with_met_asp_fang_et_al"]
ing0_L_Aspartate["L-Aspartate"]
CultureMech_007099 --> ing0_L_Aspartate
ing1_L_Methionine["L-Methionine"]
CultureMech_007099 --> ing1_L_Methionine
CHEBI_16643["CHEBI:16643"]
ing1_L_Methionine -.-> CHEBI_16643
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007099 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007099 --> ing3_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007099 --> ing4_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing5_Cobalt_chloride["Cobalt chloride"]
CultureMech_007099 --> ing5_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing5_Cobalt_chloride -.-> CHEBI_35696
ing6_Zinc_sulfate["Zinc sulfate"]
CultureMech_007099 --> ing6_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing6_Zinc_sulfate -.-> CHEBI_35176
ing7_Manganese_chloride["Manganese chloride"]
CultureMech_007099 --> ing7_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing7_Manganese_chloride -.-> CHEBI_63041
ing8_Cupric_sulfate["Cupric sulfate"]
CultureMech_007099 --> ing8_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing8_Cupric_sulfate -.-> CHEBI_23414
ing9_Sodium_citrate["Sodium citrate"]
CultureMech_007099 --> ing9_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing9_Sodium_citrate -.-> CHEBI_53258
ing10__Iron_III["'Iron(III"]
CultureMech_007099 --> ing10__Iron_III
ing11_Sodium_borate["Sodium borate"]
CultureMech_007099 --> ing11_Sodium_borate
ing12_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
CultureMech_007099 --> ing12_Molybdic_acid_ammonium_salt_tetrahydrate
CHEBI_91249["CHEBI:91249"]
ing12_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | L-Aspartate | 15.0263 MILLIMOLAR | Cross-references: KEGG:asp-L | |
| 2 |
L-Methionine
C5H11NO2S 149.215 g/mol
|
2.68078 MILLIMOLAR |
CHEBI:16643
L-methionine
|
Cross-references: KEGG:met-L |
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.00450512 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
80.3673 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
44.0898 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
8.40583e-05 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 7 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.00061931 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 8 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.000313803 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 9 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
4.00481e-05 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 10 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
15.5003 MILLIMOLAR |
CHEBI:53258
sodium citrate
|
|
| 11 | 'Iron(III | 0.000123305 MILLIMOLAR | ||
| 12 | Sodium borate | 0.000235991 MILLIMOLAR | ||
| 13 |
Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
|
3.23661e-05 MILLIMOLAR |
CHEBI:91249
ammonium molybdate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- deshpande_medium_with_met_asp_fang_et_al (CultureMech:007099)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.435695Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 206, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.129394Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007099 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.262909+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Deshpande medium with Met, Asp; Fang et al" to "deshpande_medium_with_met_asp_fang_et_al" for consistent matching |
| 2026-06-05T07:40:55.733349+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:30.044606+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9c16605e9641e4b451c0f8315c28e22886655469a854e0146750e480ca72a1cb |