Overview
- External ontology
- MEDIADB:213 (source link unavailable) — Spizizen's medium + trace elements + 0.2% glutamate + 50 ug/mL tryptophan and phenylalanine + 1% glycerol + 0.2% fumarate; Nakano et al','N
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007107["spizizens_medium_trace_elements_0_2_glutamate_50_ug_ml_tryptophan_a..."]
ing0_L_Glutamate["L-Glutamate"]
CultureMech_007107 --> ing0_L_Glutamate
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamate -.-> CHEBI_16015
ing1_Glycerol["Glycerol"]
CultureMech_007107 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_Tryptophan["Tryptophan"]
CultureMech_007107 --> ing2_Tryptophan
CHEBI_27897["CHEBI:27897"]
ing2_Tryptophan -.-> CHEBI_27897
ing3_Phenylalanine["Phenylalanine"]
CultureMech_007107 --> ing3_Phenylalanine
CHEBI_17295["CHEBI:17295"]
ing3_Phenylalanine -.-> CHEBI_17295
ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007107 --> ing4_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007107 --> ing5_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing6_Sodium_molybdate["Sodium molybdate"]
CultureMech_007107 --> ing6_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing6_Sodium_molybdate -.-> CHEBI_75215
ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007107 --> ing7_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing8_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007107 --> ing8_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing8_Magnesium_sulfate -.-> CHEBI_32599
ing9_Manganese_sulfate["Manganese sulfate"]
CultureMech_007107 --> ing9_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing9_Manganese_sulfate -.-> CHEBI_86364
ing10_Cobalt_chloride["Cobalt chloride"]
CultureMech_007107 --> ing10_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing10_Cobalt_chloride -.-> CHEBI_35696
ing11_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007107 --> ing11_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing11_Ammonium_sulfate -.-> CHEBI_62946
ing12_Cupric_chloride["Cupric chloride"]
CultureMech_007107 --> ing12_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing12_Cupric_chloride -.-> CHEBI_49553
ing13_Sodium_selenate["Sodium selenate"]
CultureMech_007107 --> ing13_Sodium_selenate
CHEBI_77775["CHEBI:77775"]
ing13_Sodium_selenate -.-> CHEBI_77775
ing14_Sodium_citrate["Sodium citrate"]
CultureMech_007107 --> ing14_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing14_Sodium_citrate -.-> CHEBI_53258
ing15__Iron_III["'Iron(III"]
CultureMech_007107 --> ing15__Iron_III
ing16_Zinc_Chloride["Zinc Chloride"]
CultureMech_007107 --> ing16_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing16_Zinc_Chloride -.-> CHEBI_49976
ing17_sodium_fumarate["sodium fumarate"]
CultureMech_007107 --> ing17_sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing17_sodium_fumarate -.-> CHEBI_115156
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamate
C5H9NO4 147.13 g/mol
|
13.5934 MILLIMOLAR |
CHEBI:16015
L-glutamic acid
|
Cross-references: KEGG:glu-L |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
108.589 MILLIMOLAR |
CHEBI:17754
glycerol
|
Cross-references: KEGG:glyc |
| 3 |
Tryptophan
C11H12N2O2 204.229 g/mol
|
0.244822 MILLIMOLAR |
CHEBI:27897
tryptophan
|
|
| 4 |
Phenylalanine
C9H11NO2 165.192 g/mol
|
0.302682 MILLIMOLAR |
CHEBI:17295
L-phenylalanine
|
|
| 5 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.049556 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 6 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
80.3673 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.00247985 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 8 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
44.0898 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.811458 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 10 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.0044828 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 11 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.0046211 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 12 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
15.1355 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 13 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.0025222 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 14 |
Sodium selenate
2Na.O4Se 188.936 g/mol
|
0.00248756 MILLIMOLAR |
CHEBI:77775
sodium selenate
|
|
| 15 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
3.4002 MILLIMOLAR |
CHEBI:53258
sodium citrate
|
|
| 16 | 'Iron(III | 0.04994 MILLIMOLAR | ||
| 17 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.012471 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
|
| 18 |
sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
12.4969 MILLIMOLAR |
CHEBI:115156
disodium fumarate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- spizizens_medium_trace_elements_0_2_glutamate_50_ug_ml_tryptophan_and_phenylalanine_1_glycerol_0_2_fumarate_nakano_et_al_n (CultureMech:007107)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.530014Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 213, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.187171Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007107 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.325750+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Spizizen's medium + trace elements + 0.2% glutamate + 50 ug/mL tryptophan and phenylalanine + 1% glycerol + 0.2% fumarate; Nakano et al','N" to "spizizens_medium_trace_elements_0_2_glutamate_50_ug_ml_tryptophan_and_phenylalanine_1_glycerol_0_2_fumarate_nakano_et_al_n" for consistent matching |
| 2026-06-05T07:41:46.480880+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:56.213616+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 36e6ed32d7c69e20edb7b5b5e3e4a4f1a1494000814a7e8edf105110315eab5b |