Overview
- External ontology
- MEDIADB:22 (source link unavailable) — Liquid MA minimal media + beta-D-glucose
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007125["liquid_ma_minimal_media_beta_d_glucose"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007125 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007125 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Boric_acid["Boric acid"]
CultureMech_007125 --> ing2_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing2_Boric_acid -.-> CHEBI_33118
ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007125 --> ing3_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing4_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007125 --> ing4_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing4_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_007125 --> ing5_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
ing6_Nickel_chloride["Nickel chloride"]
CultureMech_007125 --> ing6_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing6_Nickel_chloride -.-> CHEBI_34887
ing7_Sodium_molybdate["Sodium molybdate"]
CultureMech_007125 --> ing7_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing7_Sodium_molybdate -.-> CHEBI_75215
ing8_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007125 --> ing8_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing8_Magnesium_sulfate -.-> CHEBI_32599
ing9_Ammonium_chloride["Ammonium chloride"]
CultureMech_007125 --> ing9_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing9_Ammonium_chloride -.-> CHEBI_31206
ing10_Cobalt_chloride["Cobalt chloride"]
CultureMech_007125 --> ing10_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing10_Cobalt_chloride -.-> CHEBI_35696
ing11_Manganese_chloride["Manganese chloride"]
CultureMech_007125 --> ing11_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing11_Manganese_chloride -.-> CHEBI_63041
ing12_Cupric_chloride["Cupric chloride"]
CultureMech_007125 --> ing12_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing12_Cupric_chloride -.-> CHEBI_49553
ing13_Sodium_selenite["Sodium selenite"]
CultureMech_007125 --> ing13_Sodium_selenite
CHEBI_48843["CHEBI:48843"]
ing13_Sodium_selenite -.-> CHEBI_48843
ing14__Iron_III["'Iron(III"]
CultureMech_007125 --> ing14__Iron_III
ing15_Zinc_Chloride["Zinc Chloride"]
CultureMech_007125 --> ing15_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing15_Zinc_Chloride -.-> CHEBI_49976
ing16__Chromium_III["'Chromium(III"]
CultureMech_007125 --> ing16__Chromium_III
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
25.0 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.45 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Boric acid
H3BO3 61.833 g/mol
|
0.0003 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 4 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
25.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
31.0 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 6 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.041 MILLIMOLAR |
CHEBI:44557
nitrilotriacetic acid
|
|
| 7 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
0.0003 MILLIMOLAR |
CHEBI:34887
nickel dichloride
|
|
| 8 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.0003 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 9 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 10 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
18.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 11 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.0003 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 12 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.001 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 13 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.0003 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 14 |
Sodium selenite
2Na.O3Se 172.937 g/mol
|
0.0003 MILLIMOLAR |
CHEBI:48843
disodium selenite
|
|
| 15 | 'Iron(III | 0.003 MILLIMOLAR | ||
| 16 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.001 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
|
| 17 | 'Chromium(III | 0.0003 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- liquid_ma_minimal_media_beta_d_glucose (CultureMech:007125)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:42.701935Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 22, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.333295Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007125 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.484106+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Liquid MA minimal media + beta-D-glucose" to "liquid_ma_minimal_media_beta_d_glucose" for consistent matching |
| 2026-06-05T07:41:11.250201+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:40.342266+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1451b5efde4cea91aca9d301a7045234df1c3a7b00077fabaf3a4b864c22240a |