m9_with_6_03_zinc_acetate_and_05_cysteine_hcl_park_et_al (CultureMech:007140)

Overview

External ontology

MEDIADB:243 (source link unavailable) — M9 with 6.03% zinc acetate and .05% cysteine HCl; Park et al

Applications

Cultivation of genome-sequenced organisms
Metabolic modeling
Systems biology research

Synonyms

{'name': 'm9_with_0_67_zinc_acetate_and_05_cysteine_hcl_park_et_al', 'source': 'unknown', 'source_id': 'MEDIADB:241', 'original_category': 'bacterial'}
{'name': 'm9_with_3_35_zinc_acetate_and_05_cysteine_hcl_park_et_al', 'source': 'unknown', 'source_id': 'MEDIADB:242', 'original_category': 'bacterial'}

High-metal flag

True

Notes

Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007140["m9_with_6_03_zinc_acetate_and_05_cysteine_hcl_park_et_al"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007140 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007140 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007140 --> ing2_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007140 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_007140 --> ing4_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing4_Cysteine_HCl -.-> CHEBI_91247
    ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007140 --> ing5_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing6_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007140 --> ing6_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing6_Magnesium_sulfate -.-> CHEBI_32599
    ing7_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007140 --> ing7_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing7_Ammonium_chloride -.-> CHEBI_31206
    ing8_Zinc_Acetate["Zinc Acetate"]
    CultureMech_007140 --> ing8_Zinc_Acetate

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	D-Glucose C6H12O6 180.156 g/mol	11.1012 MILLIMOLAR	CHEBI:17634 D-glucose	Cross-references: KEGG:glc_D
2	Calcium chloride anhydrous Ca.2Cl 110.984 g/mol	0.1 MILLIMOLAR	CHEBI:3312 calcium dichloride
3	Dibasic sodium phosphate HO4P.2Na 141.958 g/mol	42.2654 MILLIMOLAR	CHEBI:34683 disodium hydrogenphosphate
4	Sodium chloride Cl.Na 58.443 g/mol	8.55578 MILLIMOLAR	CHEBI:26710 sodium chloride
5	Cysteine-HCl C3H7NO2S.HCl 157.622 g/mol	3.17219 MILLIMOLAR	CHEBI:91247 L-cysteine hydrochloride
6	Potassium dihydrogen phosphate H2O4P.K 136.084 g/mol	22.0449 MILLIMOLAR	CHEBI:63036 potassium dihydrogen phosphate
7	Magnesium sulfate Mg.O4S 120.368 g/mol	1.0 MILLIMOLAR	CHEBI:32599 magnesium sulfate
8	Ammonium chloride Cl.H4N 53.492 g/mol	18.6947 MILLIMOLAR	CHEBI:31206 ammonium chloride
9	Zinc Acetate	30.5239 MILLIMOLAR

Preparation

Dissolve all ingredients in distilled water to specified concentrations
Adjust pH if specified in original formulation
Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

m9_with_0_67_zinc_acetate_and_05_cysteine_hcl_park_et_al (CultureMech:007138)
m9_with_3_35_zinc_acetate_and_05_cysteine_hcl_park_et_al (CultureMech:007139)
m9_with_6_03_zinc_acetate_and_05_cysteine_hcl_park_et_al (CultureMech:007140)

Timestamp	Curator	Action	Notes
2026-01-27T07:21:44.835765Z	mediadb-import	Imported from MediaDB	Source: MediaDB (Institute for Systems Biology), Medium ID: 243, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.406839Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007140
2026-03-17T06:30:13.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.563303+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "M9 with 6.03% zinc acetate and .05% cysteine HCl; Park et al" to "m9_with_6_03_zinc_acetate_and_05_cysteine_hcl_park_et_al" for consistent matching
2026-06-05T07:41:12.260707+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:41.317072+00:00	recipe-merger	Merged 3 duplicate recipes into canonical record	Sources: m9_with_0_67_zinc_acetate_and_05_cysteine_hcl_park_et_al, m9_with_3_35_zinc_acetate_and_05_cysteine_hcl_park_et_al, m9_with_6_03_zinc_acetate_and_05_cysteine_hcl_park_et_al
2026-08-06T03:05:41.317117+00:00	merge_recipes.py	MERGED_RECIPES	merged 3 source recipe(s) on fingerprint b8eeabd66b532b83ab69ea1731d76dd41d4bb5655e4aebe31280e4acb2ef616f