Overview
- External ontology
- MEDIADB:257 (source link unavailable) — Modified HMM; Ozergin-Ulgen et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007155["modified_hmm_ozergin_ulgen_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007155 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Tromethamine["Tromethamine"]
CultureMech_007155 --> ing1_Tromethamine
CHEBI_9754["CHEBI:9754"]
ing1_Tromethamine -.-> CHEBI_9754
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007155 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Boric_acid["Boric acid"]
CultureMech_007155 --> ing3_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing3_Boric_acid -.-> CHEBI_33118
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007155 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Sodium_sulfate["Sodium sulfate"]
CultureMech_007155 --> ing5_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing5_Sodium_sulfate -.-> CHEBI_32149
ing6_Sodium_molybdate["Sodium molybdate"]
CultureMech_007155 --> ing6_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing6_Sodium_molybdate -.-> CHEBI_75215
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_007155 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
ing8_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007155 --> ing8_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing8_Magnesium_sulfate -.-> CHEBI_32599
ing9_Zinc_sulfate["Zinc sulfate"]
CultureMech_007155 --> ing9_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing9_Zinc_sulfate -.-> CHEBI_35176
ing10_Manganese_chloride["Manganese chloride"]
CultureMech_007155 --> ing10_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing10_Manganese_chloride -.-> CHEBI_63041
ing11_Cupric_chloride["Cupric chloride"]
CultureMech_007155 --> ing11_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing11_Cupric_chloride -.-> CHEBI_49553
ing12__Iron_III["'Iron(III"]
CultureMech_007155 --> ing12__Iron_III
ing13_Zinc_Chloride["Zinc Chloride"]
CultureMech_007155 --> ing13_Zinc_Chloride
CHEBI_49976["CHEBI:49976"]
ing13_Zinc_Chloride -.-> CHEBI_49976
ing14_Sodium_iodide["Sodium iodide"]
CultureMech_007155 --> ing14_Sodium_iodide
ing15_Sodium_nitrate["Sodium nitrate"]
CultureMech_007155 --> ing15_Sodium_nitrate
CHEBI_63005["CHEBI:63005"]
ing15_Sodium_nitrate -.-> CHEBI_63005
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
21.0924 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Tromethamine
C4H11NO3 121.136 g/mol
|
9.90589 MILLIMOLAR |
CHEBI:9754
tris
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.00108626 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Boric acid
H3BO3 61.833 g/mol
|
0.00501375 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
11.4811 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
35.2014 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 7 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.0010002 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
|
85.5578 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 9 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
4.05729 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 10 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.619066 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 11 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.00455014 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 12 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.00249296 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 13 | 'Iron(III | 0.0540999 MILLIMOLAR | ||
| 14 |
Zinc Chloride
2Cl.Zn 136.296 g/mol
|
0.0149653 MILLIMOLAR |
CHEBI:49976
zinc dichloride
|
|
| 15 | Sodium iodide | 0.0027687 MILLIMOLAR | ||
| 16 |
Sodium nitrate
NO3.Na 84.994 g/mol
|
52.9445 MILLIMOLAR |
CHEBI:63005
sodium nitrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_hmm_ozergin_ulgen_et_al (CultureMech:007155)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.976281Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 257, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.494877Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007155 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.657565+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified HMM; Ozergin-Ulgen et al" to "modified_hmm_ozergin_ulgen_et_al" for consistent matching |
| 2026-06-05T07:41:36.727957+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.418073+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b7e5af7869a15fe24a07435e2a0f9141e19eec79841b6f9cf93aab977c4dc0e8 |