Overview
- External ontology
- MEDIADB:259 (source link unavailable) — Liquid mm with arabinose; hodgson et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007157["liquid_mm_with_arabinose_hodgson_et_al"]
ing0_L_Asparagine["L-Asparagine"]
CultureMech_007157 --> ing0_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing0_L_Asparagine -.-> CHEBI_17196
ing1_L_Arabinose["L-Arabinose"]
CultureMech_007157 --> ing1_L_Arabinose
ing2_Ethylene_glycol["Ethylene glycol"]
CultureMech_007157 --> ing2_Ethylene_glycol
CHEBI_30742["CHEBI:30742"]
ing2_Ethylene_glycol -.-> CHEBI_30742
ing3_Potassium_hydroxide["Potassium hydroxide"]
CultureMech_007157 --> ing3_Potassium_hydroxide
CHEBI_32035["CHEBI:32035"]
ing3_Potassium_hydroxide -.-> CHEBI_32035
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007157 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007157 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007157 --> ing6_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing6_Ferrous_sulfate -.-> CHEBI_75832
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
3.78444 MILLIMOLAR |
CHEBI:17196
L-asparagine
|
Cross-references: KEGG:asn-L |
| 2 | L-Arabinose | 10.0 MILLIMOLAR | Cross-references: KEGG:arab-L | |
| 3 |
Ethylene glycol
C2H6O2 62.068 g/mol
|
0.134257 MILLIMOLAR |
CHEBI:30742
ethylene glycol
|
|
| 4 |
Potassium hydroxide
HO.K 56.105 g/mol
|
5.34706 MILLIMOLAR |
CHEBI:32035
potassium hydroxide
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
2.87026 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.811458 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 7 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0359648 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- liquid_mm_with_arabinose_hodgson_et_al (CultureMech:007157)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.996498Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 259, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.504671Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007157 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.668084+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Liquid mm with arabinose; hodgson et al" to "liquid_mm_with_arabinose_hodgson_et_al" for consistent matching |
| 2026-06-05T07:41:11.308841+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:40.416752+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 973601f166fd335b230b6b1f2034446bb91cbe1d81de3323f5a96cb1480453a3 |