Overview
- External ontology
- MEDIADB:274 (source link unavailable) — Bm in fructose; hodgson et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007174["bm_in_fructose_hodgson_et_al"]
ing0_D_Fructose["D-Fructose"]
CultureMech_007174 --> ing0_D_Fructose
CHEBI_15824["CHEBI:15824"]
ing0_D_Fructose -.-> CHEBI_15824
ing1_Ethylene_glycol["Ethylene glycol"]
CultureMech_007174 --> ing1_Ethylene_glycol
CHEBI_30742["CHEBI:30742"]
ing1_Ethylene_glycol -.-> CHEBI_30742
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007174 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007174 --> ing3_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing4_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007174 --> ing4_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing4_Magnesium_sulfate -.-> CHEBI_32599
ing5_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007174 --> ing5_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing5_Ferrous_sulfate -.-> CHEBI_75832
ing6_Zinc_sulfate["Zinc sulfate"]
CultureMech_007174 --> ing6_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing6_Zinc_sulfate -.-> CHEBI_35176
ing7_Manganese_chloride["Manganese chloride"]
CultureMech_007174 --> ing7_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing7_Manganese_chloride -.-> CHEBI_63041
ing8_Ammonium_nitrate["Ammonium nitrate"]
CultureMech_007174 --> ing8_Ammonium_nitrate
CHEBI_63038["CHEBI:63038"]
ing8_Ammonium_nitrate -.-> CHEBI_63038
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Fructose
C6H12O6 180.156 g/mol
|
50.0 MILLIMOLAR |
CHEBI:15824
D-fructose
|
Cross-references: KEGG:fru |
| 2 |
Ethylene glycol
C2H6O2 62.068 g/mol
|
80.5542 MILLIMOLAR |
CHEBI:30742
ethylene glycol
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.00901024 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
0.015 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.43437 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 6 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.00359648 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 7 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.0034779 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 8 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.0044829 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 9 |
Ammonium nitrate
H4N.NO3 80.043 g/mol
|
24.9838 MILLIMOLAR |
CHEBI:63038
ammonium nitrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bm_in_fructose_hodgson_et_al (CultureMech:007174)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.138523Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 274, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.585988Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007174 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.757082+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Bm in fructose; hodgson et al" to "bm_in_fructose_hodgson_et_al" for consistent matching |
| 2026-06-05T07:40:51.215344+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.679150+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3906dc936641e8411157c8c2e6d003ffd8d6f3de7a913c2e1bcd7ad640e7b3bc |