Overview
- External ontology
- MEDIADB:277 (source link unavailable) — Ecoli bl21 medium; park et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007177["ecoli_bl21_medium_park_et_al"]
ing0_Citrate["Citrate"]
CultureMech_007177 --> ing0_Citrate
CHEBI_16947["CHEBI:16947"]
ing0_Citrate -.-> CHEBI_16947
ing1_beta_D_Glucose["beta-D-Glucose"]
CultureMech_007177 --> ing1_beta_D_Glucose
ing2__Fe_III["'Fe(III"]
CultureMech_007177 --> ing2__Fe_III
ing3_Boric_acid["Boric acid"]
CultureMech_007177 --> ing3_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing3_Boric_acid -.-> CHEBI_33118
ing4_Sodium_molybdate["Sodium molybdate"]
CultureMech_007177 --> ing4_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing4_Sodium_molybdate -.-> CHEBI_75215
ing5_Thiamine_HCl["Thiamine HCl"]
CultureMech_007177 --> ing5_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing5_Thiamine_HCl -.-> CHEBI_49105
ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007177 --> ing6_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing7_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007177 --> ing7_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing7_Magnesium_sulfate -.-> CHEBI_32599
ing8_Cobalt_chloride["Cobalt chloride"]
CultureMech_007177 --> ing8_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing8_Cobalt_chloride -.-> CHEBI_35696
ing9_Manganese_chloride["Manganese chloride"]
CultureMech_007177 --> ing9_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing9_Manganese_chloride -.-> CHEBI_63041
ing10_Cupric_chloride["Cupric chloride"]
CultureMech_007177 --> ing10_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing10_Cupric_chloride -.-> CHEBI_49553
ing11_Ammonium_phosphate["Ammonium phosphate"]
CultureMech_007177 --> ing11_Ammonium_phosphate
ing12_Sodium_EDTA["Sodium EDTA"]
CultureMech_007177 --> ing12_Sodium_EDTA
ing13_Zinc_Acetate["Zinc Acetate"]
CultureMech_007177 --> ing13_Zinc_Acetate
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Citrate
C6H5O7 189.099 g/mol
|
8.8485 MILLIMOLAR |
CHEBI:16947
citrate(3-)
|
Cross-references: KEGG:cit |
| 2 | beta-D-Glucose | 27.753 MILLIMOLAR | ||
| 3 | 'Fe(III | 0.24496 MILLIMOLAR | Cross-references: KEGG:fe3dcit | |
| 4 |
Boric acid
H3BO3 61.833 g/mol
|
0.04852 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 5 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.10333 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 6 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.01491 MILLIMOLAR |
CHEBI:49105
thiamine hydrochloride
|
|
| 7 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
97.7323 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
4.86874 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 9 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.019254 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 10 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.08875 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 11 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.008799 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 12 | Ammonium phosphate | 30.287 MILLIMOLAR | ||
| 13 | Sodium EDTA | 0.02257 MILLIMOLAR | ||
| 14 | Zinc Acetate | 0.03645 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ecoli_bl21_medium_park_et_al (CultureMech:007177)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.166875Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 277, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.601639Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007177 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.774438+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ecoli bl21 medium; park et al" to "ecoli_bl21_medium_park_et_al" for consistent matching |
| 2026-06-05T07:40:59.296413+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:33.284893+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4865ff043fe992fe86299757fde501a996bbc780fb628fbaed36f1245d990826 |