aam

CultureMech:007193 bacterial DEFINED LIQUID
Original name: AAM-

Overview

External ontology
MEDIADB:292 (source link unavailable) — AAM-
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007193["aam"]
    ing0_Potassium_chloride["Potassium chloride"]
    CultureMech_007193 --> ing0_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing0_Potassium_chloride -.-> CHEBI_32588
    ing1_Sodium_chloride["Sodium chloride"]
    CultureMech_007193 --> ing1_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing1_Sodium_chloride -.-> CHEBI_26710
    ing2_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007193 --> ing2_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing2_Magnesium_sulfate -.-> CHEBI_32599
    ing3_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007193 --> ing3_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing3_Ammonium_sulfate -.-> CHEBI_62946
    ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007193 --> ing4_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007193 --> ing5_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing6_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007193 --> ing6_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing6_Ferrous_sulfate -.-> CHEBI_75832
    ing7_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007193 --> ing7_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing7_Manganese_sulfate -.-> CHEBI_86364
    ing8_Tromethamine["Tromethamine"]
    CultureMech_007193 --> ing8_Tromethamine
    CHEBI_9754["CHEBI:9754"]
    ing8_Tromethamine -.-> CHEBI_9754
    ing9_D_Glucose["D-Glucose"]
    CultureMech_007193 --> ing9_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing9_D_Glucose -.-> CHEBI_17634
    ing10_L_Arginine["L-Arginine"]
    CultureMech_007193 --> ing10_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing10_L_Arginine -.-> CHEBI_16467
    ing11_L_Proline["L-Proline"]
    CultureMech_007193 --> ing11_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing11_L_Proline -.-> CHEBI_17203
    ing12_D_Glutamate["D-Glutamate"]
    CultureMech_007193 --> ing12_D_Glutamate
    ing13_L_Valine["L-Valine"]
    CultureMech_007193 --> ing13_L_Valine
    CHEBI_16414["CHEBI:16414"]
    ing13_L_Valine -.-> CHEBI_16414
    ing14_L_Threonine["L-Threonine"]
    CultureMech_007193 --> ing14_L_Threonine
    CHEBI_16857["CHEBI:16857"]
    ing14_L_Threonine -.-> CHEBI_16857
    ing15_L_Phenylalanine["L-Phenylalanine"]
    CultureMech_007193 --> ing15_L_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing15_L_Phenylalanine -.-> CHEBI_17295
    ing16_L_Leucine["L-Leucine"]
    CultureMech_007193 --> ing16_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing16_L_Leucine -.-> CHEBI_15603
    ing17_L_Cysteine["L-Cysteine"]
    CultureMech_007193 --> ing17_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing17_L_Cysteine -.-> CHEBI_17561
    ing18_Biotin["Biotin"]
    CultureMech_007193 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_Thiamine["Thiamine"]
    CultureMech_007193 --> ing19_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing19_Thiamine -.-> CHEBI_18385
    ing20_Nicotinate["Nicotinate"]
    CultureMech_007193 --> ing20_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinate -.-> CHEBI_15940
    ing21_Pantothenate["Pantothenate"]
    CultureMech_007193 --> ing21_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing21_Pantothenate -.-> CHEBI_16454

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Potassium chloride
Cl.K 74.551 g/mol
40.2 MILLIMOLAR CHEBI:32588
potassium chloride
2 Sodium chloride
Cl.Na 58.443 g/mol
163.0 MILLIMOLAR CHEBI:26710
sodium chloride
3 Magnesium sulfate
Mg.O4S 120.368 g/mol
5.28 MILLIMOLAR CHEBI:32599
magnesium sulfate
4 Ammonium sulfate
2H4N.O4S 132.141 g/mol
30.3 MILLIMOLAR CHEBI:62946
ammonium sulfate
5 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.15 MILLIMOLAR CHEBI:3312
calcium dichloride
6 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
1.03 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
7 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.0216 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
8 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.0368 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
9 Tromethamine
C4H11NO3 121.136 g/mol
100.0 MILLIMOLAR CHEBI:9754
tris
10 D-Glucose
C6H12O6 180.156 g/mol
27.8 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
11 L-Arginine
C6H14N4O2 174.204 g/mol
0.718 MILLIMOLAR CHEBI:16467
L-arginine
 Cross-references: KEGG:arg-L
12 L-Proline
C5H9NO2 115.132 g/mol
1.74 MILLIMOLAR CHEBI:17203
L-proline
 Cross-references: KEGG:pro-L
13 D-Glutamate 1.71 MILLIMOLAR Cross-references: KEGG:glu-D
14 L-Valine
C5H11NO2 117.148 g/mol
1.28 MILLIMOLAR CHEBI:16414
L-valine
 Cross-references: KEGG:val-L
15 L-Threonine
C4H9NO3 119.12 g/mol
1.26 MILLIMOLAR CHEBI:16857
L-threonine
 Cross-references: KEGG:thr-L
16 L-Phenylalanine
C9H11NO2 165.192 g/mol
0.908 MILLIMOLAR CHEBI:17295
L-phenylalanine
 Cross-references: KEGG:phe-L
17 L-Leucine
C6H13NO2 131.175 g/mol
1.14 MILLIMOLAR CHEBI:15603
L-leucine
 Cross-references: KEGG:leu-L
18 L-Cysteine
C3H7NO2S 121.161 g/mol
0.66 MILLIMOLAR CHEBI:17561
L-cysteine
 Cross-references: KEGG:cys-L
19 Biotin
C10H16N2O3S 244.316 g/mol
0.000409 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
20 Thiamine
C12H17N4OS 265.362 g/mol
0.00665 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
21 Nicotinate
C6H5NO2 123.111 g/mol
0.0162 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
22 Pantothenate
C9H16NO5 218.229 g/mol
0.0074 MILLIMOLAR CHEBI:16454
pantothenate
 Cross-references: KEGG:pnto-R

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:45.289337Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 292, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:37.627911Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:48.909226Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:46.725846Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007193
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.906093+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AAM-" to "aam" for consistent matching
2026-06-05T07:40:46.514789+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:21.314490+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 71c3d2489b02c6aa1ed9ed038d33fa70394e71dfaae4a50aecbbf2b70222793e