minimal_medium_teusink_et_al

CultureMech:007202 bacterial DEFINED LIQUID
Original name: Minimal Medium; Teusink et al

Overview

External ontology
MEDIADB:2 (source link unavailable) — Minimal Medium; Teusink et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007202["minimal_medium_teusink_et_al"]
    ing0_L_Glutamate["L-Glutamate"]
    CultureMech_007202 --> ing0_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing0_L_Glutamate -.-> CHEBI_16015
    ing1_D_Glucose["D-Glucose"]
    CultureMech_007202 --> ing1_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing1_D_Glucose -.-> CHEBI_17634
    ing2_L_Arginine["L-Arginine"]
    CultureMech_007202 --> ing2_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing2_L_Arginine -.-> CHEBI_16467
    ing3_Ascorbate["Ascorbate"]
    CultureMech_007202 --> ing3_Ascorbate
    ing4_L_Methionine["L-Methionine"]
    CultureMech_007202 --> ing4_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing4_L_Methionine -.-> CHEBI_16643
    ing5_L_Tryptophan["L-Tryptophan"]
    CultureMech_007202 --> ing5_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing5_L_Tryptophan -.-> CHEBI_16828
    ing6_L_Phenylalanine["L-Phenylalanine"]
    CultureMech_007202 --> ing6_L_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing6_L_Phenylalanine -.-> CHEBI_17295
    ing7_L_Tyrosine["L-Tyrosine"]
    CultureMech_007202 --> ing7_L_Tyrosine
    CHEBI_17895["CHEBI:17895"]
    ing7_L_Tyrosine -.-> CHEBI_17895
    ing8_Biotin["Biotin"]
    CultureMech_007202 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_L_Leucine["L-Leucine"]
    CultureMech_007202 --> ing9_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing9_L_Leucine -.-> CHEBI_15603
    ing10_L_Valine["L-Valine"]
    CultureMech_007202 --> ing10_L_Valine
    CHEBI_16414["CHEBI:16414"]
    ing10_L_Valine -.-> CHEBI_16414
    ing11_L_Threonine["L-Threonine"]
    CultureMech_007202 --> ing11_L_Threonine
    CHEBI_16857["CHEBI:16857"]
    ing11_L_Threonine -.-> CHEBI_16857
    ing12_Nicotinate["Nicotinate"]
    CultureMech_007202 --> ing12_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinate -.-> CHEBI_15940
    ing13_Riboflavin["Riboflavin"]
    CultureMech_007202 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_L_Isoleucine["L-Isoleucine"]
    CultureMech_007202 --> ing14_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing14_L_Isoleucine -.-> CHEBI_17191
    ing15_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007202 --> ing15_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing15_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007202 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345
    ing17_Pyridoxamine_HCl["Pyridoxamine HCl"]
    CultureMech_007202 --> ing17_Pyridoxamine_HCl
    CHEBI_131531["CHEBI:131531"]
    ing17_Pyridoxamine_HCl -.-> CHEBI_131531
    ing18_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_007202 --> ing18_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_HCl -.-> CHEBI_30961
    ing19_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_007202 --> ing19_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing19_Cysteine_HCl -.-> CHEBI_91247
    ing20_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007202 --> ing20_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing20_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing21_Sodium_acetate["Sodium acetate"]
    CultureMech_007202 --> ing21_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing21_Sodium_acetate -.-> CHEBI_32954
    ing22_Ammonium_citrate["Ammonium citrate"]
    CultureMech_007202 --> ing22_Ammonium_citrate

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 L-Glutamate
C5H9NO4 147.13 g/mol
3.39836 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
2 D-Glucose
C6H12O6 180.156 g/mol
55.5062 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
3 L-Arginine
C6H14N4O2 174.204 g/mol
0.71757 MILLIMOLAR CHEBI:16467
L-arginine
 Cross-references: KEGG:arg-L
4 Ascorbate 2.83897 MILLIMOLAR Cross-references: KEGG:ascb-L
5 L-Methionine
C5H11NO2S 149.215 g/mol
0.83775 MILLIMOLAR CHEBI:16643
L-methionine
 Cross-references: KEGG:met-L
6 L-Tryptophan
C11H12N2O2 204.229 g/mol
0.24482 MILLIMOLAR CHEBI:16828
L-tryptophan
 Cross-references: KEGG:trp-L
7 L-Phenylalanine
C9H11NO2 165.192 g/mol
1.66475 MILLIMOLAR CHEBI:17295
L-phenylalanine
 Cross-references: KEGG:phe-L
8 L-Tyrosine
C9H11NO3 181.191 g/mol
1.37977 MILLIMOLAR CHEBI:17895
L-tyrosine
 Cross-references: KEGG:tyr-L
9 Biotin
C10H16N2O3S 244.316 g/mol
0.01203 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
10 L-Leucine
C6H13NO2 131.175 g/mol
3.62125 MILLIMOLAR CHEBI:15603
L-leucine
 Cross-references: KEGG:leu-L
11 L-Valine
C5H11NO2 117.148 g/mol
2.77422 MILLIMOLAR CHEBI:16414
L-valine
 Cross-references: KEGG:val-L
12 L-Threonine
C4H9NO3 119.12 g/mol
1.88885 MILLIMOLAR CHEBI:16857
L-threonine
 Cross-references: KEGG:thr-L
13 Nicotinate
C6H5NO2 123.111 g/mol
0.00812 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
14 Riboflavin
C17H20N4O6 376.369 g/mol
0.00266 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
15 L-Isoleucine
C6H13NO2 131.175 g/mol
1.60098 MILLIMOLAR CHEBI:17191
L-isoleucine
 Cross-references: KEGG:ile-L
16 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
5.74053 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.00456 MILLIMOLAR CHEBI:31345
Calcium pantothenate
18 Pyridoxamine HCl
C8H12N2O2.HCl 204.657 g/mol
0.02443 MILLIMOLAR CHEBI:131531
pyridoxamine hydrochloride
19 Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
0.00973 MILLIMOLAR CHEBI:30961
pyridoxine hydrochloride
20 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
0.82477 MILLIMOLAR CHEBI:91247
L-cysteine hydrochloride
21 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
36.7415 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
22 Sodium acetate
C2H3O2.Na 82.034 g/mol
12.1901 MILLIMOLAR CHEBI:32954
sodium acetate
23 Ammonium citrate 2.65269 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:42.501358Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 2, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:08.905984Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:14.413764Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:46.836709Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007202
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.018474+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Minimal Medium; Teusink et al" to "minimal_medium_teusink_et_al" for consistent matching
2026-06-05T07:41:34.713399+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:48.511306+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-06T03:05:46.399919+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 36d7105ab7eac3bbf98fa0fe9f8b061c10884384be5e32cb8b277763f0bf146f