chemically_defined_fermentation_medium_20g_glycerol_12_5g_msg

CultureMech:007226 bacterial DEFINED LIQUID
Original name: 'Chemically Defined Fermentation Medium (20g glycerol + 12.5g MSG

Overview

External ontology
MEDIADB:327 (source link unavailable) — 'Chemically Defined Fermentation Medium (20g glycerol + 12.5g MSG
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007226["chemically_defined_fermentation_medium_20g_glycerol_12_5g_msg"]
    ing0_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007226 --> ing0_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing0_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing1_Glycerol["Glycerol"]
    CultureMech_007226 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_Boric_acid["Boric acid"]
    CultureMech_007226 --> ing2_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing2_Boric_acid -.-> CHEBI_33118
    ing3_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007226 --> ing3_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing3_Ferrous_sulfate -.-> CHEBI_75832
    ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007226 --> ing4_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing5_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007226 --> ing5_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing5_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing6_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007226 --> ing6_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing6_Manganese_sulfate -.-> CHEBI_86364
    ing7_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007226 --> ing7_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing7_Magnesium_sulfate -.-> CHEBI_32599
    ing8_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007226 --> ing8_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing8_Zinc_sulfate -.-> CHEBI_35176
    ing9_Cupric_chloride["Cupric chloride"]
    CultureMech_007226 --> ing9_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing9_Cupric_chloride -.-> CHEBI_49553
    ing10_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
    CultureMech_007226 --> ing10_Molybdic_acid_ammonium_salt_tetrahydrate
    CHEBI_91249["CHEBI:91249"]
    ing10_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
    ing11_Sodium_Glutamate["Sodium Glutamate"]
    CultureMech_007226 --> ing11_Sodium_Glutamate
    CHEBI_64220["CHEBI:64220"]
    ing11_Sodium_Glutamate -.-> CHEBI_64220

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
28.71 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
2 Glycerol
C3H8O3 92.094 g/mol
217.18 MILLIMOLAR CHEBI:17754
glycerol
 Cross-references: KEGG:glyc
3 Boric acid
H3BO3 61.833 g/mol
0.1779 MILLIMOLAR CHEBI:33118
boric acid
4 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.7194 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
5 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
36.74 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
6 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.136 MILLIMOLAR CHEBI:3312
calcium dichloride
7 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.4483 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
8 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.4057 MILLIMOLAR CHEBI:32599
magnesium sulfate
9 Zinc sulfate
O4S.Zn 161.453 g/mol
0.6955 MILLIMOLAR CHEBI:35176
zinc sulfate
10 Cupric chloride
Cl2Cu 134.452 g/mol
0.02933 MILLIMOLAR CHEBI:49553
copper(II) chloride
11 Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
0.004045 MILLIMOLAR CHEBI:91249
ammonium molybdate
12 Sodium Glutamate
C5H8NO4.Na 169.112 g/mol
66.8 MILLIMOLAR CHEBI:64220
monosodium glutamate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:45.575648Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 327, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:24.938399Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:47.015790Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007226
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.204576+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Chemically Defined Fermentation Medium (20g glycerol + 12.5g MSG" to "chemically_defined_fermentation_medium_20g_glycerol_12_5g_msg" for consistent matching
2026-06-05T07:40:53.109313+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:27.295169+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 24f7bfcec8dedb62fed9f0352fbee31e950366155fe755eb3783ac1ae8520444