Overview
- External ontology
- MEDIADB:329 (source link unavailable) — 'Chemically Defined Fermentation Medium (20g glucose + 20g lactose +12.5g MSG
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007228["chemically_defined_fermentation_medium_20g_glucose_20g_lactose_12_5..."]
ing0_Boric_acid["Boric acid"]
CultureMech_007228 --> ing0_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing0_Boric_acid -.-> CHEBI_33118
ing1_Lactose["Lactose"]
CultureMech_007228 --> ing1_Lactose
CHEBI_17716["CHEBI:17716"]
ing1_Lactose -.-> CHEBI_17716
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007228 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Manganese_sulfate["Manganese sulfate"]
CultureMech_007228 --> ing3_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing3_Manganese_sulfate -.-> CHEBI_86364
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007228 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Glucose["Glucose"]
CultureMech_007228 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007228 --> ing6_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing6_Magnesium_sulfate -.-> CHEBI_32599
ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007228 --> ing7_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing8_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007228 --> ing8_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing8_Ferrous_sulfate -.-> CHEBI_75832
ing9_Zinc_sulfate["Zinc sulfate"]
CultureMech_007228 --> ing9_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing9_Zinc_sulfate -.-> CHEBI_35176
ing10_Cupric_chloride["Cupric chloride"]
CultureMech_007228 --> ing10_Cupric_chloride
CHEBI_49553["CHEBI:49553"]
ing10_Cupric_chloride -.-> CHEBI_49553
ing11_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
CultureMech_007228 --> ing11_Molybdic_acid_ammonium_salt_tetrahydrate
CHEBI_91249["CHEBI:91249"]
ing11_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
ing12_Sodium_Glutamate["Sodium Glutamate"]
CultureMech_007228 --> ing12_Sodium_Glutamate
CHEBI_64220["CHEBI:64220"]
ing12_Sodium_Glutamate -.-> CHEBI_64220
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Boric acid
H3BO3 61.833 g/mol
|
0.1779 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 2 |
Lactose
C12H22O11 342.297 g/mol
|
58.43 MILLIMOLAR |
CHEBI:17716
lactose
|
Cross-references: KEGG:lcts |
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.136 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.4483 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
28.71 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
111.01 MILLIMOLAR |
CHEBI:17234
glucose
|
|
| 7 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.4057 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
36.74 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.7194 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 10 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.6955 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 11 |
Cupric chloride
Cl2Cu 134.452 g/mol
|
0.02933 MILLIMOLAR |
CHEBI:49553
copper(II) chloride
|
|
| 12 |
Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
|
0.004045 MILLIMOLAR |
CHEBI:91249
ammonium molybdate
|
|
| 13 |
Sodium Glutamate
C5H8NO4.Na 169.112 g/mol
|
66.8 MILLIMOLAR |
CHEBI:64220
monosodium glutamate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- chemically_defined_fermentation_medium_20g_glucose_20g_lactose_12_5g_msg (CultureMech:007228)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.596641Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 329, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:11.299954Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:47.029238Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007228 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.218245+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'Chemically Defined Fermentation Medium (20g glucose + 20g lactose +12.5g MSG" to "chemically_defined_fermentation_medium_20g_glucose_20g_lactose_12_5g_msg" for consistent matching |
| 2026-06-05T07:40:53.101041+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:27.286085+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 916d98e1423f1de801e0c608fb2816ca66077437f9e0b0e446f70d00b166efac |