Overview
- External ontology
- MEDIADB:32 (source link unavailable) — Minimal Medium; Lostroh et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007229["minimal_medium_lostroh_et_al"]
ing0_Succinate["Succinate"]
CultureMech_007229 --> ing0_Succinate
CHEBI_26806["CHEBI:26806"]
ing0_Succinate -.-> CHEBI_26806
ing1_EDTA["EDTA"]
CultureMech_007229 --> ing1_EDTA
CHEBI_4735["CHEBI:4735"]
ing1_EDTA -.-> CHEBI_4735
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007229 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007229 --> ing3_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing4_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007229 --> ing4_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing4_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_007229 --> ing5_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
ing6_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007229 --> ing6_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing6_Magnesium_sulfate -.-> CHEBI_32599
ing7_Manganese_sulfate["Manganese sulfate"]
CultureMech_007229 --> ing7_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing7_Manganese_sulfate -.-> CHEBI_86364
ing8_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007229 --> ing8_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing8_Ferrous_sulfate -.-> CHEBI_75832
ing9_Zinc_sulfate["Zinc sulfate"]
CultureMech_007229 --> ing9_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing9_Zinc_sulfate -.-> CHEBI_35176
ing10_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007229 --> ing10_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing10_Ammonium_sulfate -.-> CHEBI_62946
ing11_Cupric_sulfate["Cupric sulfate"]
CultureMech_007229 --> ing11_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing11_Cupric_sulfate -.-> CHEBI_23414
ing12_Sodium_borate["Sodium borate"]
CultureMech_007229 --> ing12_Sodium_borate
ing13_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
CultureMech_007229 --> ing13_Molybdic_acid_ammonium_salt_tetrahydrate
CHEBI_91249["CHEBI:91249"]
ing13_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
ing14_Cobalt_nitrate["Cobalt nitrate"]
CultureMech_007229 --> ing14_Cobalt_nitrate
CHEBI_86209["CHEBI:86209"]
ing14_Cobalt_nitrate -.-> CHEBI_86209
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Succinate | 10.0 MILLIMOLAR |
CHEBI:26806
succinate
|
Cross-references: KEGG:succ |
| 2 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.008554 MILLIMOLAR |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.603712 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
50.0 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
50.0 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 6 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.151 MILLIMOLAR |
CHEBI:44557
nitrilotriacetic acid
|
|
| 7 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
4.8186 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.009111 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.03114 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 10 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.038082 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 11 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
7.5677 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 12 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.0015698 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 13 | Sodium borate | 0.0004641 MILLIMOLAR | ||
| 14 |
Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
|
0.00016183 MILLIMOLAR |
CHEBI:91249
ammonium molybdate
|
|
| 15 |
Cobalt nitrate
Co.2NO3 182.941 g/mol
|
0.000852146 MILLIMOLAR |
CHEBI:86209
cobalt dinitrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- minimal_medium_lostroh_et_al (CultureMech:007229)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:42.800303Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 32, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:47.035957Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007229 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.225347+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Minimal Medium; Lostroh et al" to "minimal_medium_lostroh_et_al" for consistent matching |
| 2026-06-05T07:41:34.692221+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:46.369931+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 660d6dbf0484951029f08464d349c628558c9bf2e489b796cd27a499ebe832d9 |