Overview
- External ontology
- MEDIADB:33 (source link unavailable) — CDM/LP
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007240["cdm_lp"]
ing0__["?"]
CultureMech_007240 --> ing0__
ing1_L_Glutamate["L-Glutamate"]
CultureMech_007240 --> ing1_L_Glutamate
CHEBI_16015["CHEBI:16015"]
ing1_L_Glutamate -.-> CHEBI_16015
ing2_D_Glucose["D-Glucose"]
CultureMech_007240 --> ing2_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing2_D_Glucose -.-> CHEBI_17634
ing3_Glycine["Glycine"]
CultureMech_007240 --> ing3_Glycine
CHEBI_15428["CHEBI:15428"]
ing3_Glycine -.-> CHEBI_15428
ing4_L_Aspartate["L-Aspartate"]
CultureMech_007240 --> ing4_L_Aspartate
ing5_Glutathione["Glutathione"]
CultureMech_007240 --> ing5_Glutathione
CHEBI_16856["CHEBI:16856"]
ing5_Glutathione -.-> CHEBI_16856
ing6_L_Glutamine["L-Glutamine"]
CultureMech_007240 --> ing6_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing6_L_Glutamine -.-> CHEBI_18050
ing7_L_Serine["L-Serine"]
CultureMech_007240 --> ing7_L_Serine
CHEBI_17115["CHEBI:17115"]
ing7_L_Serine -.-> CHEBI_17115
ing8_Ascorbate["Ascorbate"]
CultureMech_007240 --> ing8_Ascorbate
ing9_L_Methionine["L-Methionine"]
CultureMech_007240 --> ing9_L_Methionine
CHEBI_16643["CHEBI:16643"]
ing9_L_Methionine -.-> CHEBI_16643
ing10_L_Tryptophan["L-Tryptophan"]
CultureMech_007240 --> ing10_L_Tryptophan
CHEBI_16828["CHEBI:16828"]
ing10_L_Tryptophan -.-> CHEBI_16828
ing11_L_Phenylalanine["L-Phenylalanine"]
CultureMech_007240 --> ing11_L_Phenylalanine
CHEBI_17295["CHEBI:17295"]
ing11_L_Phenylalanine -.-> CHEBI_17295
ing12_L_Tyrosine["L-Tyrosine"]
CultureMech_007240 --> ing12_L_Tyrosine
CHEBI_17895["CHEBI:17895"]
ing12_L_Tyrosine -.-> CHEBI_17895
ing13_L_Cysteine["L-Cysteine"]
CultureMech_007240 --> ing13_L_Cysteine
CHEBI_17561["CHEBI:17561"]
ing13_L_Cysteine -.-> CHEBI_17561
ing14_Uracil["Uracil"]
CultureMech_007240 --> ing14_Uracil
CHEBI_17568["CHEBI:17568"]
ing14_Uracil -.-> CHEBI_17568
ing15_Choline["Choline"]
CultureMech_007240 --> ing15_Choline
CHEBI_15354["CHEBI:15354"]
ing15_Choline -.-> CHEBI_15354
ing16_Biotin["Biotin"]
CultureMech_007240 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_D_Ribose["D-Ribose"]
CultureMech_007240 --> ing17_D_Ribose
ing18_L_Leucine["L-Leucine"]
CultureMech_007240 --> ing18_L_Leucine
CHEBI_15603["CHEBI:15603"]
ing18_L_Leucine -.-> CHEBI_15603
ing19_D_Alanine["D-Alanine"]
CultureMech_007240 --> ing19_D_Alanine
ing20_myo_Inositol["myo-Inositol"]
CultureMech_007240 --> ing20_myo_Inositol
CHEBI_17268["CHEBI:17268"]
ing20_myo_Inositol -.-> CHEBI_17268
ing21_L_Proline["L-Proline"]
CultureMech_007240 --> ing21_L_Proline
CHEBI_17203["CHEBI:17203"]
ing21_L_Proline -.-> CHEBI_17203
ing22_Nicotinamide["Nicotinamide"]
CultureMech_007240 --> ing22_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing22_Nicotinamide -.-> CHEBI_17154
ing23_Thymine["Thymine"]
CultureMech_007240 --> ing23_Thymine
CHEBI_17821["CHEBI:17821"]
ing23_Thymine -.-> CHEBI_17821
ing24_L_Valine["L-Valine"]
CultureMech_007240 --> ing24_L_Valine
CHEBI_16414["CHEBI:16414"]
ing24_L_Valine -.-> CHEBI_16414
ing25_Cholesterol["Cholesterol"]
CultureMech_007240 --> ing25_Cholesterol
CHEBI_16113["CHEBI:16113"]
ing25_Cholesterol -.-> CHEBI_16113
ing26_L_Threonine["L-Threonine"]
CultureMech_007240 --> ing26_L_Threonine
CHEBI_16857["CHEBI:16857"]
ing26_L_Threonine -.-> CHEBI_16857
ing27_Nicotinate["Nicotinate"]
CultureMech_007240 --> ing27_Nicotinate
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinate -.-> CHEBI_15940
ing28_Riboflavin["Riboflavin"]
CultureMech_007240 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Hypoxanthine["Hypoxanthine"]
CultureMech_007240 --> ing29_Hypoxanthine
CHEBI_17368["CHEBI:17368"]
ing29_Hypoxanthine -.-> CHEBI_17368
CultureMech_007240 --> more["...34 more"]
Ingredients (64)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.00036 MILLIMOLAR | |||
| 2 |
L-Glutamate
C5H9NO4 147.13 g/mol
|
0.34 MILLIMOLAR |
CHEBI:16015
L-glutamic acid
|
Cross-references: KEGG:glu-L |
| 3 |
D-Glucose
C6H12O6 180.156 g/mol
|
13.3 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 4 |
Glycine
C2H5NO2 75.067 g/mol
|
0.28 MILLIMOLAR |
CHEBI:15428
glycine
|
Cross-references: KEGG:gly |
| 5 | L-Aspartate | 0.26 MILLIMOLAR | Cross-references: KEGG:asp-L | |
| 6 |
Glutathione
C10H17N3O6S 307.328 g/mol
|
0.0036 MILLIMOLAR |
CHEBI:16856
glutathione
|
Cross-references: KEGG:gthrd |
| 7 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
2.3 MILLIMOLAR |
CHEBI:18050
L-glutamine
|
Cross-references: KEGG:gln-L |
| 8 |
L-Serine
C3H7NO3 105.093 g/mol
|
0.42 MILLIMOLAR |
CHEBI:17115
L-serine
|
Cross-references: KEGG:ser-L |
| 9 | Ascorbate | 6e-05 MILLIMOLAR | Cross-references: KEGG:ascb-L | |
| 10 |
L-Methionine
C5H11NO2S 149.215 g/mol
|
0.14 MILLIMOLAR |
CHEBI:16643
L-methionine
|
Cross-references: KEGG:met-L |
| 11 |
L-Tryptophan
C11H12N2O2 204.229 g/mol
|
0.046 MILLIMOLAR |
CHEBI:16828
L-tryptophan
|
Cross-references: KEGG:trp-L |
| 12 |
L-Phenylalanine
C9H11NO2 165.192 g/mol
|
0.16 MILLIMOLAR |
CHEBI:17295
L-phenylalanine
|
Cross-references: KEGG:phe-L |
| 13 |
L-Tyrosine
C9H11NO3 181.191 g/mol
|
0.17 MILLIMOLAR |
CHEBI:17895
L-tyrosine
|
Cross-references: KEGG:tyr-L |
| 14 |
L-Cysteine
C3H7NO2S 121.161 g/mol
|
0.6 MILLIMOLAR |
CHEBI:17561
L-cysteine
|
Cross-references: KEGG:cys-L |
| 15 |
Uracil
C4H4N2O2 112.088 g/mol
|
0.00062 MILLIMOLAR |
CHEBI:17568
uracil
|
Cross-references: KEGG:ura |
| 16 |
Choline
C5H14NO 104.173 g/mol
|
0.024 MILLIMOLAR |
CHEBI:15354
choline
|
Cross-references: KEGG:chol |
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0015 MILLIMOLAR |
CHEBI:15956
biotin
|
Cross-references: KEGG:btn |
| 18 | D-Ribose | 0.66 MILLIMOLAR | Cross-references: KEGG:rib-D | |
| 19 |
L-Leucine
C6H13NO2 131.175 g/mol
|
0.6 MILLIMOLAR |
CHEBI:15603
L-leucine
|
Cross-references: KEGG:leu-L |
| 20 | D-Alanine | 0.11 MILLIMOLAR | Cross-references: KEGG:ala-D | |
| 21 |
myo-Inositol
C6H12O6 180.156 g/mol
|
0.00025 MILLIMOLAR |
CHEBI:17268
myo-inositol
|
Cross-references: KEGG:inost |
| 22 |
L-Proline
C5H9NO2 115.132 g/mol
|
0.2 MILLIMOLAR |
CHEBI:17203
L-proline
|
Cross-references: KEGG:pro-L |
| 23 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.0094 MILLIMOLAR |
CHEBI:17154
nicotinamide
|
Cross-references: KEGG:ncam |
| 24 |
Thymine
C5H6N2O2 126.115 g/mol
|
0.00048 MILLIMOLAR |
CHEBI:17821
thymine
|
Cross-references: KEGG:thym |
| 25 |
L-Valine
C5H11NO2 117.148 g/mol
|
0.27 MILLIMOLAR |
CHEBI:16414
L-valine
|
Cross-references: KEGG:val-L |
| 26 |
Cholesterol
C27H46O 386.664 g/mol
|
0.0001 MILLIMOLAR |
CHEBI:16113
cholesterol
|
Cross-references: KEGG:chsterol |
| 27 |
L-Threonine
C4H9NO3 119.12 g/mol
|
0.29 MILLIMOLAR |
CHEBI:16857
L-threonine
|
Cross-references: KEGG:thr-L |
| 28 |
Nicotinate
C6H5NO2 123.111 g/mol
|
4e-05 MILLIMOLAR |
CHEBI:15940
nicotinic acid
|
Cross-references: KEGG:nac |
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.00056 MILLIMOLAR |
CHEBI:17015
riboflavin
|
Cross-references: KEGG:ribflv |
| 30 |
Hypoxanthine
C5H4N4O 136.114 g/mol
|
0.00052 MILLIMOLAR |
CHEBI:17368
hypoxanthine
|
Cross-references: KEGG:hxan |
| 31 |
Xanthine
C5H4N4O2 152.111 g/mol
|
0.0005 MILLIMOLAR |
CHEBI:15318
xanthine
|
Cross-references: KEGG:xan |
| 32 |
L-Isoleucine
C6H13NO2 131.175 g/mol
|
0.48 MILLIMOLAR |
CHEBI:17191
L-isoleucine
|
Cross-references: KEGG:ile-L |
| 33 | Retinol | 7e-05 MILLIMOLAR | Cross-references: KEGG:retinol | |
| 34 |
Folate
C19H19N7O6 441.404 g/mol
|
0.003 MILLIMOLAR |
CHEBI:27470
folic acid
|
Cross-references: KEGG:fol |
| 35 |
4-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.009 MILLIMOLAR |
CHEBI:17836
4-aminobenzoate
|
Cross-references: KEGG:4abz |
| 36 |
Pantothenate
C9H16NO5 218.229 g/mol
|
0.0009 MILLIMOLAR |
CHEBI:16454
pantothenate
|
Cross-references: KEGG:pnto-R |
| 37 | 4-Hydroxy-L-proline | 0.17 MILLIMOLAR | ||
| 38 | Deoxyribose | 0.74 MILLIMOLAR | Cross-references: KEGG:drib | |
| 39 | alpha-Tocopherol | 1e-05 MILLIMOLAR | Cross-references: KEGG:avite1 | |
| 40 | 'Cyanocob(III | 0.004 MILLIMOLAR | ||
| 41 |
Menadione
C11H8O2 172.183 g/mol
|
1e-05 MILLIMOLAR |
CHEBI:28869
menadione
|
Cross-references: KEGG:mndn |
| 42 | Vitamin D3 | 5e-05 MILLIMOLAR | Cross-references: KEGG:vitd3 | |
| 43 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.72 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 44 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.003053 MILLIMOLAR |
CHEBI:53426
polysorbate 80
|
|
| 45 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
24.0 MILLIMOLAR |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 46 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
6.22 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 47 |
Asparagine
C4H8N2O3 132.119 g/mol
|
0.36 MILLIMOLAR |
CHEBI:22653
asparagine
|
|
| 48 |
Sodium chloride
Cl.Na 58.443 g/mol
|
139.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 49 |
Potassium chloride
Cl.K 74.551 g/mol
|
7.0 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 50 |
Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
|
0.0054 MILLIMOLAR |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 51 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.00034 MILLIMOLAR |
CHEBI:49105
thiamine hydrochloride
|
|
| 52 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.00012 MILLIMOLAR |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 53 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
0.09 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 54 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.12 MILLIMOLAR |
CHEBI:32954
sodium acetate
|
|
| 55 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.61 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 56 |
Pyridoxal HCl
C8H9NO3.HCl 203.625 g/mol
|
0.001 MILLIMOLAR |
CHEBI:131529
pyridoxal hydrochloride
|
|
| 57 | Ferric nitrate | 0.00036 MILLIMOLAR | ||
| 58 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
7.7 MILLIMOLAR |
CHEBI:50385
hemin
|
|
| 59 | Arginine HCl | 1.9 MILLIMOLAR | ||
| 60 | Histidine HCl | 0.13 MILLIMOLAR | ||
| 61 | Lysine-HCl | 0.32 MILLIMOLAR | ||
| 62 | Adenosine sulfate | 0.0086 MILLIMOLAR | ||
| 63 | Guanine HCl | 0.00032 MILLIMOLAR | ||
| 64 | HEPES | 20.0 MILLIMOLAR |
CHEBI:46756
HEPES
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- cdm_lp (CultureMech:007240)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:42.809402Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 33, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:21.045290Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:29.348397Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:47.116813Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007240 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.311201+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CDM/LP" to "cdm_lp" for consistent matching |
| 2026-06-05T07:40:52.804984+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 37 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.186877+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:26.958507+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint aee07a9289ff579754c7a409fdc01994add7357d19739c4ce3c4696c8ac38147 |
| 2026-08-21T01:39:51.992536+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22/G24) | Re-grounded 1 term reference(s) to the correct CHEBI (audit G21/G22/G24; see reports/chebi_grounding_audit.md): CHEBI:140435→CHEBI:16113 ×1. |