chemically_defined_fermentation_medium_45g_glucose_12_5g_isoleucine

CultureMech:007243 bacterial DEFINED LIQUID
Original name: 'Chemically Defined Fermentation Medium (45g glucose + 12.5g isoleucine

Overview

External ontology
MEDIADB:342 (source link unavailable) — 'Chemically Defined Fermentation Medium (45g glucose + 12.5g isoleucine
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007243["chemically_defined_fermentation_medium_45g_glucose_12_5g_isoleucine"]
    ing0_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007243 --> ing0_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing0_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing1_Glucose["Glucose"]
    CultureMech_007243 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Boric_acid["Boric acid"]
    CultureMech_007243 --> ing2_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing2_Boric_acid -.-> CHEBI_33118
    ing3_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007243 --> ing3_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing3_Ferrous_sulfate -.-> CHEBI_75832
    ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007243 --> ing4_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing5_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007243 --> ing5_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing5_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing6_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007243 --> ing6_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing6_Manganese_sulfate -.-> CHEBI_86364
    ing7_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007243 --> ing7_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing7_Magnesium_sulfate -.-> CHEBI_32599
    ing8_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007243 --> ing8_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing8_Zinc_sulfate -.-> CHEBI_35176
    ing9_Cupric_chloride["Cupric chloride"]
    CultureMech_007243 --> ing9_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing9_Cupric_chloride -.-> CHEBI_49553
    ing10_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
    CultureMech_007243 --> ing10_Molybdic_acid_ammonium_salt_tetrahydrate
    CHEBI_91249["CHEBI:91249"]
    ing10_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
    ing11_Isoleucine["Isoleucine"]
    CultureMech_007243 --> ing11_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing11_Isoleucine -.-> CHEBI_17191

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
28.71 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
2 Glucose
C6H12O6 180.156 g/mol
249.8 MILLIMOLAR CHEBI:17234
glucose
3 Boric acid
H3BO3 61.833 g/mol
0.1779 MILLIMOLAR CHEBI:33118
boric acid
4 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.7194 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
5 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
36.74 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
6 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.136 MILLIMOLAR CHEBI:3312
calcium dichloride
7 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.4483 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
8 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.4057 MILLIMOLAR CHEBI:32599
magnesium sulfate
9 Zinc sulfate
O4S.Zn 161.453 g/mol
0.6955 MILLIMOLAR CHEBI:35176
zinc sulfate
10 Cupric chloride
Cl2Cu 134.452 g/mol
0.02933 MILLIMOLAR CHEBI:49553
copper(II) chloride
11 Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
0.004045 MILLIMOLAR CHEBI:91249
ammonium molybdate
12 Isoleucine
C6H13NO2 131.175 g/mol
95.3 MILLIMOLAR CHEBI:17191
L-isoleucine

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:45.736533Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 342, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:47.141298Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007243
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.337872+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Chemically Defined Fermentation Medium (45g glucose + 12.5g isoleucine" to "chemically_defined_fermentation_medium_45g_glucose_12_5g_isoleucine" for consistent matching
2026-06-05T07:40:53.149101+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:27.338789+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b97149ebfa0e41e51c9e43444230a7231be502b0bd0f2c945a5e471e720dfcce