Overview
- External ontology
- MEDIADB:345 (source link unavailable) — 'Mogensen Minimal Medium (Ethanol
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007246["mogensen_minimal_medium_ethanol"]
ing0_Boric_acid["Boric acid"]
CultureMech_007246 --> ing0_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing0_Boric_acid -.-> CHEBI_33118
ing1_Ethanol["Ethanol"]
CultureMech_007246 --> ing1_Ethanol
CHEBI_16236["CHEBI:16236"]
ing1_Ethanol -.-> CHEBI_16236
ing2_Tromethamine["Tromethamine"]
CultureMech_007246 --> ing2_Tromethamine
CHEBI_9754["CHEBI:9754"]
ing2_Tromethamine -.-> CHEBI_9754
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007246 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Sodium_molybdate["Sodium molybdate"]
CultureMech_007246 --> ing4_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing4_Sodium_molybdate -.-> CHEBI_75215
ing5_4_Aminobenzoate["4-Aminobenzoate"]
CultureMech_007246 --> ing5_4_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing5_4_Aminobenzoate -.-> CHEBI_17836
ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007246 --> ing6_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing7_Potassium_chloride["Potassium chloride"]
CultureMech_007246 --> ing7_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing7_Potassium_chloride -.-> CHEBI_32588
ing8_Ammonium_chloride["Ammonium chloride"]
CultureMech_007246 --> ing8_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing8_Ammonium_chloride -.-> CHEBI_31206
ing9_Manganese_chloride["Manganese chloride"]
CultureMech_007246 --> ing9_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing9_Manganese_chloride -.-> CHEBI_63041
ing10_Cupric_sulfate["Cupric sulfate"]
CultureMech_007246 --> ing10_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing10_Cupric_sulfate -.-> CHEBI_23414
ing11_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007246 --> ing11_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing11_Ferrous_sulfate -.-> CHEBI_75832
ing12_Sodium_EDTA["Sodium EDTA"]
CultureMech_007246 --> ing12_Sodium_EDTA
ing13_Cobalt_chloride["Cobalt chloride"]
CultureMech_007246 --> ing13_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing13_Cobalt_chloride -.-> CHEBI_35696
ing14_Zinc_sulfate["Zinc sulfate"]
CultureMech_007246 --> ing14_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing14_Zinc_sulfate -.-> CHEBI_35176
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Boric acid
H3BO3 61.833 g/mol
|
0.1779 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 2 |
Ethanol
C2H6O 46.069 g/mol
|
625.1 MILLIMOLAR |
CHEBI:16236
ethanol
|
Cross-references: KEGG:etoh |
| 3 |
Tromethamine
C4H11NO3 121.136 g/mol
|
49.7 MILLIMOLAR |
CHEBI:9754
tris
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.16 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.006199 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 6 |
4-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.07292 MILLIMOLAR |
CHEBI:17836
4-aminobenzoate
|
Cross-references: KEGG:4abz |
| 7 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
11.17 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
Potassium chloride
Cl.K 74.551 g/mol
|
6.975 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 9 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
138.3 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 10 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.02526 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 11 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.006408 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 12 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.01798 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 13 | Sodium EDTA | 0.1315 MILLIMOLAR | ||
| 14 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.007145 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 15 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.07651 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mogensen_minimal_medium_ethanol (CultureMech:007246)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.763517Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 345, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:37.956093Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:47.159817Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007246 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.358228+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'Mogensen Minimal Medium (Ethanol" to "mogensen_minimal_medium_ethanol" for consistent matching |
| 2026-06-05T07:41:38.101433+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:50.109800+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 058385bf1b99ddd56285e6f381b9d961bfd2cdb01a167e014ced7628df16ebe9 |