Overview
- External ontology
- MEDIADB:346 (source link unavailable) — 'Batch Cultivation Medium (for A28
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007247["batch_cultivation_medium_for_a28"]
ing0_Sodium_chloride["Sodium chloride"]
CultureMech_007247 --> ing0_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing0_Sodium_chloride -.-> CHEBI_26710
ing1_Xylose["Xylose"]
CultureMech_007247 --> ing1_Xylose
CHEBI_18222["CHEBI:18222"]
ing1_Xylose -.-> CHEBI_18222
ing2_Nickel_chloride["Nickel chloride"]
CultureMech_007247 --> ing2_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing2_Nickel_chloride -.-> CHEBI_34887
ing3_Zinc_sulfate["Zinc sulfate"]
CultureMech_007247 --> ing3_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing3_Zinc_sulfate -.-> CHEBI_35176
ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007247 --> ing4_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing5_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007247 --> ing5_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing5_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing6_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007247 --> ing6_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing6_Ferrous_sulfate -.-> CHEBI_75832
ing7_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007247 --> ing7_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing7_Magnesium_sulfate -.-> CHEBI_32599
ing8_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007247 --> ing8_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing8_Ammonium_sulfate -.-> CHEBI_62946
ing9_Cupric_sulfate["Cupric sulfate"]
CultureMech_007247 --> ing9_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing9_Cupric_sulfate -.-> CHEBI_23414
ing10_4_Aminobenzoate["4-Aminobenzoate"]
CultureMech_007247 --> ing10_4_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing10_4_Aminobenzoate -.-> CHEBI_17836
ing11_Biotin["Biotin"]
CultureMech_007247 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium chloride
Cl.Na 58.443 g/mol
|
34.22 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 2 |
Xylose
C5H10O5 150.13 g/mol
|
66.61 MILLIMOLAR |
CHEBI:18222
xylose
|
|
| 3 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
0.002104 MILLIMOLAR |
CHEBI:34887
nickel dichloride
|
|
| 4 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.07969 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 5 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
22.04 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
1.36 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 7 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.08122 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 8 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
8.115 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 9 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
113.5 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 10 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.01001 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 11 |
4-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.007292 MILLIMOLAR |
CHEBI:17836
4-aminobenzoate
|
Cross-references: KEGG:4abz |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004093 MILLIMOLAR |
CHEBI:15956
biotin
|
Cross-references: KEGG:btn |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- batch_cultivation_medium_for_a28 (CultureMech:007247)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.772853Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 346, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:47.166227Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007247 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.365543+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'Batch Cultivation Medium (for A28" to "batch_cultivation_medium_for_a28" for consistent matching |
| 2026-06-05T07:40:50.611659+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:24.845972+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 50dbb089c12e7ac953828f0288db99e60586873f683bacccff97e54028799743 |