rdm_base_medium_vitamins_maltose_1_g_l_nh4cl

CultureMech:007285 bacterial DEFINED LIQUID
Original name: 'RDM Base Medium + Vitamins + Maltose (1 g/L NH4Cl

Overview

External ontology
MEDIADB:381 (source link unavailable) — 'RDM Base Medium + Vitamins + Maltose (1 g/L NH4Cl
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Synonyms
  • {'name': 'rdm_base_medium_vitamins_maltose_0_5_g_l_nh4cl', 'source': 'unknown', 'source_id': 'MEDIADB:382', 'original_category': 'bacterial'}
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007285["rdm_base_medium_vitamins_maltose_1_g_l_nh4cl"]
    ing0_Riboflavin["Riboflavin"]
    CultureMech_007285 --> ing0_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing0_Riboflavin -.-> CHEBI_17015
    ing1_Biotin["Biotin"]
    CultureMech_007285 --> ing1_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing1_Biotin -.-> CHEBI_15956
    ing2_Nicotinate["Nicotinate"]
    CultureMech_007285 --> ing2_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing2_Nicotinate -.-> CHEBI_15940
    ing3_Vitamin_B12["Vitamin B12"]
    CultureMech_007285 --> ing3_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing3_Vitamin_B12 -.-> CHEBI_176843
    ing4_Folate["Folate"]
    CultureMech_007285 --> ing4_Folate
    CHEBI_27470["CHEBI:27470"]
    ing4_Folate -.-> CHEBI_27470
    ing5_Maltose["Maltose"]
    CultureMech_007285 --> ing5_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing5_Maltose -.-> CHEBI_17306
    ing6_Lipoate["Lipoate"]
    CultureMech_007285 --> ing6_Lipoate
    CHEBI_16494["CHEBI:16494"]
    ing6_Lipoate -.-> CHEBI_16494
    ing7_4_Aminobenzoate["4-Aminobenzoate"]
    CultureMech_007285 --> ing7_4_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing7_4_Aminobenzoate -.-> CHEBI_17836
    ing8_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007285 --> ing8_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing8_Magnesium_chloride -.-> CHEBI_86345
    ing9_Resazurin["Resazurin"]
    CultureMech_007285 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Potassium_iodide["Potassium iodide"]
    CultureMech_007285 --> ing10_Potassium_iodide
    CHEBI_8346["CHEBI:8346"]
    ing10_Potassium_iodide -.-> CHEBI_8346
    ing11_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007285 --> ing11_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing11_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing12_Boric_acid["Boric acid"]
    CultureMech_007285 --> ing12_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing12_Boric_acid -.-> CHEBI_33118
    ing13_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007285 --> ing13_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing13_Thiamine_HCl -.-> CHEBI_49105
    ing14_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_007285 --> ing14_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing14_Pyridoxine_HCl -.-> CHEBI_30961
    ing15_Sodium_sulfide["Sodium sulfide"]
    CultureMech_007285 --> ing15_Sodium_sulfide
    CHEBI_76208["CHEBI:76208"]
    ing15_Sodium_sulfide -.-> CHEBI_76208
    ing16_Sodium_tungstate["Sodium tungstate"]
    CultureMech_007285 --> ing16_Sodium_tungstate
    CHEBI_63940["CHEBI:63940"]
    ing16_Sodium_tungstate -.-> CHEBI_63940
    ing17_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007285 --> ing17_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing17_Sodium_sulfate -.-> CHEBI_32149
    ing18_Sodium_chloride["Sodium chloride"]
    CultureMech_007285 --> ing18_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing18_Sodium_chloride -.-> CHEBI_26710
    ing19_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007285 --> ing19_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing19_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing20_Sodium_bromide["Sodium bromide"]
    CultureMech_007285 --> ing20_Sodium_bromide
    CHEBI_63004["CHEBI:63004"]
    ing20_Sodium_bromide -.-> CHEBI_63004
    ing21_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007285 --> ing21_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing21_Ammonium_chloride -.-> CHEBI_31206
    ing22_Strontium_chloride["Strontium chloride"]
    CultureMech_007285 --> ing22_Strontium_chloride
    CHEBI_36383["CHEBI:36383"]
    ing22_Strontium_chloride -.-> CHEBI_36383
    ing23_Potassium_chloride["Potassium chloride"]
    CultureMech_007285 --> ing23_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing23_Potassium_chloride -.-> CHEBI_32588
    ing24_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007285 --> ing24_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 Riboflavin
C17H20N4O6 376.369 g/mol
0.0001063 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
2 Biotin
C10H16N2O3S 244.316 g/mol
8.186e-05 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
3 Nicotinate
C6H5NO2 123.111 g/mol
0.0004061 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
4 Vitamin B12 7.378e-07 MILLIMOLAR CHEBI:176843
vitamin B12
5 Folate
C19H19N7O6 441.404 g/mol
4.531e-05 MILLIMOLAR CHEBI:27470
folic acid
 Cross-references: KEGG:fol
6 Maltose
C12H22O11 342.297 g/mol
5.843 MILLIMOLAR CHEBI:17306
maltose
 Cross-references: KEGG:malt
7 Lipoate
C8H14O2S2 206.332 g/mol
0.0002423 MILLIMOLAR CHEBI:16494
lipoic acid
 Cross-references: KEGG:lipoate
8 4-Aminobenzoate
C7H6NO2 136.13 g/mol
0.0003646 MILLIMOLAR CHEBI:17836
4-aminobenzoate
 Cross-references: KEGG:4abz
9 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
4.919 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
10 Resazurin
C12H7NO4 229.191 g/mol
0.004363 MILLIMOLAR CHEBI:8806
Resazurin
11 Potassium iodide
I.K 166.002 g/mol
0.1205 MILLIMOLAR CHEBI:8346
potassium iodide
12 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
0.8038 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
13 Boric acid
H3BO3 61.833 g/mol
0.3235 MILLIMOLAR CHEBI:33118
boric acid
14 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.0001482 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
15 Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
0.0004863 MILLIMOLAR CHEBI:30961
pyridoxine hydrochloride
16 Sodium sulfide
2Na.S 78.047 g/mol
0.4164 MILLIMOLAR CHEBI:76208
sodium sulfide (anhydrous)
17 Sodium tungstate
2Na.O4W 293.816 g/mol
0.009095 MILLIMOLAR CHEBI:63940
sodium tungstate
18 Sodium sulfate
2Na.O4S 142.043 g/mol
7.04 MILLIMOLAR CHEBI:32149
sodium sulfate
19 Sodium chloride
Cl.Na 58.443 g/mol
256.7 MILLIMOLAR CHEBI:26710
sodium chloride
20 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.5102 MILLIMOLAR CHEBI:3312
calcium dichloride
21 Sodium bromide
Br.Na 102.894 g/mol
0.486 MILLIMOLAR CHEBI:63004
sodium bromide
22 Ammonium chloride
Cl.H4N 53.492 g/mol
9.348 MILLIMOLAR CHEBI:31206
ammonium chloride
23 Strontium chloride
2Cl.Sr 158.526 g/mol
0.03751 MILLIMOLAR CHEBI:36383
strontium dichloride
24 Potassium chloride
Cl.K 74.551 g/mol
4.695 MILLIMOLAR CHEBI:32588
potassium chloride
25 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0001049 MILLIMOLAR CHEBI:31345
Calcium pantothenate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T07:21:46.065602Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 381, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:38.300779Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:49.791015Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:47.406000Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007285
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.626598+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'RDM Base Medium + Vitamins + Maltose (1 g/L NH4Cl" to "rdm_base_medium_vitamins_maltose_1_g_l_nh4cl" for consistent matching
2026-06-05T07:41:44.037900+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.145985+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-06T03:05:54.469077+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: rdm_base_medium_vitamins_maltose_0_5_g_l_nh4cl, rdm_base_medium_vitamins_maltose_1_g_l_nh4cl
2026-08-06T03:05:54.469134+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 9988931b53bbfe0155feca6cac647246a13928947fa488efb1c937c7ec49b21f