Overview
- External ontology
- MEDIADB:385 (source link unavailable) — 'BG11, 0.015 mM Iron (Shcolnick 2006
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007289["bg11_0_015_mm_iron_shcolnick_2006"]
ing0_Cobalt_nitrate["Cobalt nitrate"]
CultureMech_007289 --> ing0_Cobalt_nitrate
CHEBI_86209["CHEBI:86209"]
ing0_Cobalt_nitrate -.-> CHEBI_86209
ing1_Cupric_sulfate["Cupric sulfate"]
CultureMech_007289 --> ing1_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing1_Cupric_sulfate -.-> CHEBI_23414
ing2_Sodium_nitrate["Sodium nitrate"]
CultureMech_007289 --> ing2_Sodium_nitrate
CHEBI_63005["CHEBI:63005"]
ing2_Sodium_nitrate -.-> CHEBI_63005
ing3_Manganese_chloride["Manganese chloride"]
CultureMech_007289 --> ing3_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing3_Manganese_chloride -.-> CHEBI_63041
ing4_Boric_acid["Boric acid"]
CultureMech_007289 --> ing4_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing4_Boric_acid -.-> CHEBI_33118
ing5_Sodium_molybdate["Sodium molybdate"]
CultureMech_007289 --> ing5_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing5_Sodium_molybdate -.-> CHEBI_75215
ing6_Zinc_sulfate["Zinc sulfate"]
CultureMech_007289 --> ing6_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing6_Zinc_sulfate -.-> CHEBI_35176
ing7__Fe_III["'Fe(III"]
CultureMech_007289 --> ing7__Fe_III
ing8_Citrate["Citrate"]
CultureMech_007289 --> ing8_Citrate
CHEBI_16947["CHEBI:16947"]
ing8_Citrate -.-> CHEBI_16947
ing9_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007289 --> ing9_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing9_Magnesium_sulfate -.-> CHEBI_32599
ing10_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007289 --> ing10_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing10_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing11_EDTA["EDTA"]
CultureMech_007289 --> ing11_EDTA
CHEBI_4735["CHEBI:4735"]
ing11_EDTA -.-> CHEBI_4735
ing12_sodium_silicate["sodium silicate"]
CultureMech_007289 --> ing12_sodium_silicate
CHEBI_60720["CHEBI:60720"]
ing12_sodium_silicate -.-> CHEBI_60720
ing13_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007289 --> ing13_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing13_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing14_Sodium_carbonate["Sodium carbonate"]
CultureMech_007289 --> ing14_Sodium_carbonate
CHEBI_29377["CHEBI:29377"]
ing14_Sodium_carbonate -.-> CHEBI_29377
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Cobalt nitrate
Co.2NO3 182.941 g/mol
|
0.0001697 MILLIMOLAR |
CHEBI:86209
cobalt dinitrate
|
|
| 2 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.0003164 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 3 |
Sodium nitrate
NO3.Na 84.994 g/mol
|
10.03 MILLIMOLAR |
CHEBI:63005
sodium nitrate
|
|
| 4 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.009145 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 5 |
Boric acid
H3BO3 61.833 g/mol
|
0.04626 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 6 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.001616 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 7 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.0007721 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 8 | 'Fe(III | 0.015 MILLIMOLAR | Cross-references: KEGG:fe3dcit | |
| 9 |
Citrate
C6H5O7 189.099 g/mol
|
0.03123 MILLIMOLAR |
CHEBI:16947
citrate(3-)
|
Cross-references: KEGG:cit |
| 10 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.3043 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 11 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
0.2239 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.003422 MILLIMOLAR |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 13 |
sodium silicate
2Na.O3Si 122.063 g/mol
|
0.2041 MILLIMOLAR |
CHEBI:60720
sodium silicate
|
|
| 14 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.2433 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 15 |
Sodium carbonate
CO3.2Na 105.988 g/mol
|
0.1887 MILLIMOLAR |
CHEBI:29377
sodium carbonate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bg11_0_015_mm_iron_shcolnick_2006 (CultureMech:007289)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:46.106083Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 385, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:07.188182Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:47.439889Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007289 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.665526+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'BG11, 0.015 mM Iron (Shcolnick 2006" to "bg11_0_015_mm_iron_shcolnick_2006" for consistent matching |
| 2026-06-05T07:40:50.812118+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.065126+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7fd64b201bd30f143504738ef9da1672b1337c7c5a4609e44046d2639bc164d6 |