fwafc

CultureMech:007301 bacterial DEFINED LIQUID
Original name: FWAFC

Overview

External ontology
MEDIADB:3 (source link unavailable) — FWAFC
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007301["fwafc"]
    ing0_Acetate["Acetate"]
    CultureMech_007301 --> ing0_Acetate
    CHEBI_30089["CHEBI:30089"]
    ing0_Acetate -.-> CHEBI_30089
    ing1_Biotin["Biotin"]
    CultureMech_007301 --> ing1_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing1_Biotin -.-> CHEBI_15956
    ing2_Nicotinate["Nicotinate"]
    CultureMech_007301 --> ing2_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing2_Nicotinate -.-> CHEBI_15940
    ing3_Riboflavin["Riboflavin"]
    CultureMech_007301 --> ing3_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing3_Riboflavin -.-> CHEBI_17015
    ing4_Folate["Folate"]
    CultureMech_007301 --> ing4_Folate
    CHEBI_27470["CHEBI:27470"]
    ing4_Folate -.-> CHEBI_27470
    ing5_4_Aminobenzoate["4-Aminobenzoate"]
    CultureMech_007301 --> ing5_4_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing5_4_Aminobenzoate -.-> CHEBI_17836
    ing6_Lipoate["Lipoate"]
    CultureMech_007301 --> ing6_Lipoate
    CHEBI_16494["CHEBI:16494"]
    ing6_Lipoate -.-> CHEBI_16494
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_007301 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8__Fe_III["'Fe(III"]
    CultureMech_007301 --> ing8__Fe_III
    ing9_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007301 --> ing9_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing9_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing10_Boric_acid["Boric acid"]
    CultureMech_007301 --> ing10_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing10_Boric_acid -.-> CHEBI_33118
    ing11_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_007301 --> ing11_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing11_Sodium_bicarbonate -.-> CHEBI_32139
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_007301 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_Nickel_chloride["Nickel chloride"]
    CultureMech_007301 --> ing13_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing13_Nickel_chloride -.-> CHEBI_34887
    ing14_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007301 --> ing14_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing14_Sodium_molybdate -.-> CHEBI_75215
    ing15_Sodium_chloride["Sodium chloride"]
    CultureMech_007301 --> ing15_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing15_Sodium_chloride -.-> CHEBI_26710
    ing16_Potassium_chloride["Potassium chloride"]
    CultureMech_007301 --> ing16_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing16_Potassium_chloride -.-> CHEBI_32588
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007301 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_007301 --> ing18_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_HCl -.-> CHEBI_30961
    ing19_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007301 --> ing19_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing19_Thiamine_HCl -.-> CHEBI_49105
    ing20_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007301 --> ing20_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing20_Magnesium_sulfate -.-> CHEBI_32599
    ing21_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007301 --> ing21_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing21_Manganese_sulfate -.-> CHEBI_86364
    ing22_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007301 --> ing22_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing22_Ammonium_chloride -.-> CHEBI_31206
    ing23_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007301 --> ing23_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing23_Ferrous_sulfate -.-> CHEBI_75832
    ing24_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007301 --> ing24_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing24_Cobalt_chloride -.-> CHEBI_35696
    ing25_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007301 --> ing25_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing25_Zinc_sulfate -.-> CHEBI_35176
    ing26_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
    CultureMech_007301 --> ing26_Sodium_dihydrogen_phosphate
    CHEBI_37585["CHEBI:37585"]
    ing26_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
    ing27_Sodium_selenate["Sodium selenate"]
    CultureMech_007301 --> ing27_Sodium_selenate
    CHEBI_77775["CHEBI:77775"]
    ing27_Sodium_selenate -.-> CHEBI_77775
    ing28_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007301 --> ing28_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing28_Cupric_sulfate -.-> CHEBI_23414
    ing29_Aluminum_potassium_sulfate["Aluminum potassium sulfate"]
    CultureMech_007301 --> ing29_Aluminum_potassium_sulfate
    CHEBI_86463["CHEBI:86463"]
    ing29_Aluminum_potassium_sulfate -.-> CHEBI_86463

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 Acetate
C2H3O2 59.044 g/mol
20.0 MILLIMOLAR CHEBI:30089
acetate
 Cross-references: KEGG:ac
2 Biotin
C10H16N2O3S 244.316 g/mol
8.18e-05 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
3 Nicotinate
C6H5NO2 123.111 g/mol
0.000406 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
4 Riboflavin
C17H20N4O6 376.369 g/mol
0.000133 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
5 Folate
C19H19N7O6 441.404 g/mol
4.53e-05 MILLIMOLAR CHEBI:27470
folic acid
 Cross-references: KEGG:fol
6 4-Aminobenzoate
C7H6NO2 136.13 g/mol
0.000365 MILLIMOLAR CHEBI:17836
4-aminobenzoate
 Cross-references: KEGG:4abz
7 Lipoate
C8H14O2S2 206.332 g/mol
0.000242 MILLIMOLAR CHEBI:16494
lipoic acid
 Cross-references: KEGG:lipoate
8 Vitamin B12 7.37e-07 MILLIMOLAR CHEBI:176843
vitamin B12
9 'Fe(III 57.1057 MILLIMOLAR Cross-references: KEGG:fe3dcit
10 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
6.8e-06 MILLIMOLAR CHEBI:3312
calcium dichloride
11 Boric acid
H3BO3 61.833 g/mol
1.617e-06 MILLIMOLAR CHEBI:33118
boric acid
12 Sodium bicarbonate
CHO3.Na 84.006 g/mol
29.7595 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
7.85e-05 MILLIMOLAR CHEBI:44557
nitrilotriacetic acid
14 Nickel chloride
Cl2Ni 129.599 g/mol
1.0097e-06 MILLIMOLAR CHEBI:34887
nickel dichloride
15 Sodium molybdate
MoO4.2Na 205.916 g/mol
1.0333e-06 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
16 Sodium chloride
Cl.Na 58.443 g/mol
0.000171 MILLIMOLAR CHEBI:26710
sodium chloride
17 Potassium chloride
Cl.K 74.551 g/mol
1.34136 MILLIMOLAR CHEBI:32588
potassium chloride
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.000228 MILLIMOLAR CHEBI:31345
Calcium pantothenate
19 Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
0.000486 MILLIMOLAR CHEBI:30961
pyridoxine hydrochloride
20 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.000166 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
21 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.000122 MILLIMOLAR CHEBI:32599
magnesium sulfate
22 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
2.67e-05 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
23 Ammonium chloride
Cl.H4N 53.492 g/mol
4.67362 MILLIMOLAR CHEBI:31206
ammonium chloride
24 Ferrous sulfate
Fe.O4S 151.908 g/mol
3.6e-06 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
25 Cobalt chloride
2Cl.Co 129.839 g/mol
7.7e-06 MILLIMOLAR CHEBI:35696
cobalt dichloride
26 Zinc sulfate
O4S.Zn 161.453 g/mol
6.19e-06 MILLIMOLAR CHEBI:35176
zinc sulfate
27 Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
4.35033 MILLIMOLAR CHEBI:37585
sodium dihydrogenphosphate
28 Sodium selenate
2Na.O4Se 188.936 g/mol
0.1 MILLIMOLAR CHEBI:77775
sodium selenate
29 Cupric sulfate
Cu.O4S 159.609 g/mol
4.005e-07 MILLIMOLAR CHEBI:23414
copper(II) sulfate
30 Aluminum potassium sulfate
Al.K.2O4S 258.206 g/mol
3.873e-07 MILLIMOLAR CHEBI:86463
potassium aluminium sulfate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:42.510267Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 3, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:18.181202Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:25.852760Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:47.551677Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007301
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.829339+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "FWAFC" to "fwafc" for consistent matching
2026-06-05T07:41:05.755664+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.257539+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-09T01:42:20.574706+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-08-06T03:05:35.814284+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0bb25abe5954c558558c41770222fa86559b0aeb18679f520c17768762f21867