defined_freshwater_medium_coso4

CultureMech:007317 bacterial DEFINED LIQUID
Original name: 'Defined freshwater medium (CoSO4

Overview

External ontology
MEDIADB:416 (source link unavailable) — 'Defined freshwater medium (CoSO4
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007317["defined_freshwater_medium_coso4"]
    ing0_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007317 --> ing0_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing0_Zinc_sulfate -.-> CHEBI_35176
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007317 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Nicotinate["Nicotinate"]
    CultureMech_007317 --> ing2_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing2_Nicotinate -.-> CHEBI_15940
    ing3_Potassium_chloride["Potassium chloride"]
    CultureMech_007317 --> ing3_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing3_Potassium_chloride -.-> CHEBI_32588
    ing4_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_007317 --> ing4_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing4_Sodium_bicarbonate -.-> CHEBI_32139
    ing5_Lipoate["Lipoate"]
    CultureMech_007317 --> ing5_Lipoate
    CHEBI_16494["CHEBI:16494"]
    ing5_Lipoate -.-> CHEBI_16494
    ing6_4_Aminobenzoate["4-Aminobenzoate"]
    CultureMech_007317 --> ing6_4_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing6_4_Aminobenzoate -.-> CHEBI_17836
    ing7_Acetate["Acetate"]
    CultureMech_007317 --> ing7_Acetate
    CHEBI_30089["CHEBI:30089"]
    ing7_Acetate -.-> CHEBI_30089
    ing8_Ferric_oxide["Ferric oxide"]
    CultureMech_007317 --> ing8_Ferric_oxide
    ing9_Vitamin_B12["Vitamin B12"]
    CultureMech_007317 --> ing9_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamin_B12 -.-> CHEBI_176843
    ing10_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007317 --> ing10_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing10_Cupric_sulfate -.-> CHEBI_23414
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_007317 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_Sodium_chloride["Sodium chloride"]
    CultureMech_007317 --> ing12_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing12_Sodium_chloride -.-> CHEBI_26710
    ing13_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007317 --> ing13_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing13_Sodium_molybdate -.-> CHEBI_75215
    ing14_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007317 --> ing14_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing14_Magnesium_sulfate -.-> CHEBI_32599
    ing15_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007317 --> ing15_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing15_Ferrous_sulfate -.-> CHEBI_75832
    ing16_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007317 --> ing16_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing16_Thiamine_HCl -.-> CHEBI_49105
    ing17_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007317 --> ing17_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing17_Manganese_sulfate -.-> CHEBI_86364
    ing18_Riboflavin["Riboflavin"]
    CultureMech_007317 --> ing18_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing18_Riboflavin -.-> CHEBI_17015
    ing19_Aluminum_potassium_sulfate["Aluminum potassium sulfate"]
    CultureMech_007317 --> ing19_Aluminum_potassium_sulfate
    CHEBI_86463["CHEBI:86463"]
    ing19_Aluminum_potassium_sulfate -.-> CHEBI_86463
    ing20_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
    CultureMech_007317 --> ing20_Sodium_dihydrogen_phosphate
    CHEBI_37585["CHEBI:37585"]
    ing20_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
    ing21_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007317 --> ing21_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing21_Ammonium_chloride -.-> CHEBI_31206
    ing22_Folate["Folate"]
    CultureMech_007317 --> ing22_Folate
    CHEBI_27470["CHEBI:27470"]
    ing22_Folate -.-> CHEBI_27470
    ing23_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007317 --> ing23_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Boric_acid["Boric acid"]
    CultureMech_007317 --> ing24_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing24_Boric_acid -.-> CHEBI_33118
    ing25_Biotin["Biotin"]
    CultureMech_007317 --> ing25_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_Biotin -.-> CHEBI_15956
    ing26_Cobaltous_sulfate["Cobaltous sulfate"]
    CultureMech_007317 --> ing26_Cobaltous_sulfate
    CHEBI_53470["CHEBI:53470"]
    ing26_Cobaltous_sulfate -.-> CHEBI_53470
    ing27_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_007317 --> ing27_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_HCl -.-> CHEBI_30961
    ing28_Nickel_chloride["Nickel chloride"]
    CultureMech_007317 --> ing28_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing28_Nickel_chloride -.-> CHEBI_34887

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 Zinc sulfate
O4S.Zn 161.453 g/mol
0.006194 MILLIMOLAR CHEBI:35176
zinc sulfate
2 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.687 MILLIMOLAR CHEBI:3312
calcium dichloride
3 Nicotinate
C6H5NO2 123.111 g/mol
0.0004061 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
4 Potassium chloride
Cl.K 74.551 g/mol
1.341 MILLIMOLAR CHEBI:32588
potassium chloride
5 Sodium bicarbonate
CHO3.Na 84.006 g/mol
29.76 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
6 Lipoate
C8H14O2S2 206.332 g/mol
0.0002423 MILLIMOLAR CHEBI:16494
lipoic acid
 Cross-references: KEGG:lipoate
7 4-Aminobenzoate
C7H6NO2 136.13 g/mol
0.0003646 MILLIMOLAR CHEBI:17836
4-aminobenzoate
 Cross-references: KEGG:4abz
8 Acetate
C2H3O2 59.044 g/mol
113.2 MILLIMOLAR CHEBI:30089
acetate
 Cross-references: KEGG:ac
9 Ferric oxide 100.0 MILLIMOLAR
10 Vitamin B12 7.378e-07 MILLIMOLAR CHEBI:176843
vitamin B12
11 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0004005 MILLIMOLAR CHEBI:23414
copper(II) sulfate
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.07848 MILLIMOLAR CHEBI:44557
nitrilotriacetic acid
13 Sodium chloride
Cl.Na 58.443 g/mol
0.1711 MILLIMOLAR CHEBI:26710
sodium chloride
14 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.001627 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
15 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.1217 MILLIMOLAR CHEBI:32599
magnesium sulfate
16 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.003597 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
17 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.0001482 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
18 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.02673 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
19 Riboflavin
C17H20N4O6 376.369 g/mol
0.0001329 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
20 Aluminum potassium sulfate
Al.K.2O4S 258.206 g/mol
0.0003873 MILLIMOLAR CHEBI:86463
potassium aluminium sulfate
21 Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
4.348 MILLIMOLAR CHEBI:37585
sodium dihydrogenphosphate
22 Ammonium chloride
Cl.H4N 53.492 g/mol
28.04 MILLIMOLAR CHEBI:31206
ammonium chloride
23 Folate
C19H19N7O6 441.404 g/mol
4.531e-05 MILLIMOLAR CHEBI:27470
folic acid
 Cross-references: KEGG:fol
24 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0001049 MILLIMOLAR CHEBI:31345
Calcium pantothenate
25 Boric acid
H3BO3 61.833 g/mol
0.001617 MILLIMOLAR CHEBI:33118
boric acid
26 Biotin
C10H16N2O3S 244.316 g/mol
8.186e-05 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
27 Cobaltous sulfate
Co.O4S 154.996 g/mol
0.006494 MILLIMOLAR CHEBI:53470
cobalt(2+) sulfate
28 Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
0.0004863 MILLIMOLAR CHEBI:30961
pyridoxine hydrochloride
29 Nickel chloride
Cl2Ni 129.599 g/mol
0.00101 MILLIMOLAR CHEBI:34887
nickel dichloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

TimestampCuratorActionNotes
2026-01-27T07:21:46.418392Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 416, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:17.932184Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:25.518299Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:47.744305Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007317
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:06.046616+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Defined freshwater medium (CoSO4" to "defined_freshwater_medium_coso4" for consistent matching
2026-06-05T07:40:28.575586+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:28.850177+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-09T01:42:15.680650+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G22) G22 phosphate speciation split (see reports/chebi_grounding_audit.md): re-grounded Sodium dihydrogen phosphate from CHEBI:37583 (trisodium phosphate) to CHEBI:37585 (sodium dihydrogenphosphate) on its term and mediaingredientmech_chebi_term references.
2026-08-06T03:05:03.607908+00:00 recipe-merger Merged 4 duplicate recipes into canonical record Sources: MEDIADB_416_Defined_freshwater_medium_CoSO4, MEDIADB_417_Defined_freshwater_medium_CoSO4, MEDIADB_423_Defined_freshwater_medium_CoSO4, MEDIADB_424_Defined_freshwater_medium_CoSO4
2026-08-06T03:05:03.608025+00:00 merge_recipes.py MERGED_RECIPES merged 4 source recipe(s) on fingerprint 49655c629778a35c0306fd6c3f13817292f2ab346deadad891dc2d572a5e48b2