Overview
- External ontology
- MEDIADB:472 (source link unavailable) — 'HS Medium (Methanol-Acetate
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007379["hs_medium_methanol_acetate"]
ing0_Acetate["Acetate"]
CultureMech_007379 --> ing0_Acetate
CHEBI_30089["CHEBI:30089"]
ing0_Acetate -.-> CHEBI_30089
ing1_Methanol["Methanol"]
CultureMech_007379 --> ing1_Methanol
CHEBI_17790["CHEBI:17790"]
ing1_Methanol -.-> CHEBI_17790
ing2_Sodium_chloride["Sodium chloride"]
CultureMech_007379 --> ing2_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing2_Sodium_chloride -.-> CHEBI_26710
ing3_Resazurin["Resazurin"]
CultureMech_007379 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007379 --> ing4_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing5_Sodium_sulfide["Sodium sulfide"]
CultureMech_007379 --> ing5_Sodium_sulfide
CHEBI_76208["CHEBI:76208"]
ing5_Sodium_sulfide -.-> CHEBI_76208
ing6_Cysteine_HCl["Cysteine-HCl"]
CultureMech_007379 --> ing6_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing6_Cysteine_HCl -.-> CHEBI_91247
ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007379 --> ing7_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing8_Potassium_chloride["Potassium chloride"]
CultureMech_007379 --> ing8_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing8_Potassium_chloride -.-> CHEBI_32588
ing9_Ammonium_chloride["Ammonium chloride"]
CultureMech_007379 --> ing9_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing9_Ammonium_chloride -.-> CHEBI_31206
ing10_Sodium_bicarbonate["Sodium bicarbonate"]
CultureMech_007379 --> ing10_Sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing10_Sodium_bicarbonate -.-> CHEBI_32139
ing11_Magnesium_chloride["Magnesium chloride"]
CultureMech_007379 --> ing11_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing11_Magnesium_chloride -.-> CHEBI_86345
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Acetate
C2H3O2 59.044 g/mol
|
40.0 MILLIMOLAR |
CHEBI:30089
acetate
|
Cross-references: KEGG:ac |
| 2 |
Methanol
CH4O 32.042 g/mol
|
125.0 MILLIMOLAR |
CHEBI:17790
methanol
|
Cross-references: KEGG:meoh |
| 3 |
Sodium chloride
Cl.Na 58.443 g/mol
|
400.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.004 MILLIMOLAR |
CHEBI:8806
Resazurin
|
|
| 5 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
2.0 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 6 |
Sodium sulfide
2Na.S 78.047 g/mol
|
0.4 MILLIMOLAR |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 7 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
2.8 MILLIMOLAR |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 8 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
5.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
Potassium chloride
Cl.K 74.551 g/mol
|
13.0 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 10 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
19.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 11 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
45.0 MILLIMOLAR |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
54.0 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hs_medium_methanol_acetate (CultureMech:007379)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:47.007693Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 472, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:21.536016Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:48.520440Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007379 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:06.863252+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'HS Medium (Methanol-Acetate" to "hs_medium_methanol_acetate" for consistent matching |
| 2026-06-05T07:41:08.750621+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:37.843528+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9d34fda56e810a18f7051a9334248e38b11934af735d3156cba01c295f2e7e16 |