mcn_medium_whitman

CultureMech:007380 bacterial COMPLEX LIQUID
Original name: 'McN Medium (Whitman

Overview

External ontology
MEDIADB:473 (source link unavailable) — 'McN Medium (Whitman
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/ Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_007380["mcn_medium_whitman"]
    ing0_Tryptone["Tryptone"]
    CultureMech_007380 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007380 --> ing1_Yeast_extract
    ing2_Sodium_chloride["Sodium chloride"]
    CultureMech_007380 --> ing2_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_chloride -.-> CHEBI_26710
    ing3_Sodium_sulfide["Sodium sulfide"]
    CultureMech_007380 --> ing3_Sodium_sulfide
    CHEBI_76208["CHEBI:76208"]
    ing3_Sodium_sulfide -.-> CHEBI_76208
    ing4_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_007380 --> ing4_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing4_Cysteine_HCl -.-> CHEBI_91247
    ing5_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
    CultureMech_007380 --> ing5_2_Mercaptoethanesulfonate
    CHEBI_17905["CHEBI:17905"]
    ing5_2_Mercaptoethanesulfonate -.-> CHEBI_17905
    ing6_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_007380 --> ing6_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing6_Sodium_bicarbonate -.-> CHEBI_32139
    ing7_Potassium_chloride["Potassium chloride"]
    CultureMech_007380 --> ing7_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing7_Potassium_chloride -.-> CHEBI_32588
    ing8_Resazurin["Resazurin"]
    CultureMech_007380 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_Sodium_chloride["Sodium chloride"]
    CultureMech_007380 --> ing9_Sodium_chloride
    ing9_Sodium_chloride -.-> CHEBI_26710
    ing10_2_Methylpropanoate["2-Methylpropanoate"]
    CultureMech_007380 --> ing10_2_Methylpropanoate
    ing11_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007380 --> ing11_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing11_Zinc_sulfate -.-> CHEBI_35176
    ing12_Sodium_tungstate["Sodium tungstate"]
    CultureMech_007380 --> ing12_Sodium_tungstate
    CHEBI_63940["CHEBI:63940"]
    ing12_Sodium_tungstate -.-> CHEBI_63940
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_007380 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007380 --> ing14_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing14_Manganese_sulfate -.-> CHEBI_86364
    ing15_Nickel_chloride["Nickel chloride"]
    CultureMech_007380 --> ing15_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing15_Nickel_chloride -.-> CHEBI_34887
    ing16_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007380 --> ing16_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing16_Ammonium_chloride -.-> CHEBI_31206
    ing17_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007380 --> ing17_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing17_Magnesium_sulfate -.-> CHEBI_32599
    ing18_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007380 --> ing18_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing18_Sodium_molybdate -.-> CHEBI_75215
    ing19_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007380 --> ing19_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing19_Magnesium_chloride -.-> CHEBI_86345
    ing20_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007380 --> ing20_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing20_Cobalt_chloride -.-> CHEBI_35696
    ing21_Pentanoate["Pentanoate"]
    CultureMech_007380 --> ing21_Pentanoate
    ing22_Ferrous_ammonium_sulfate["Ferrous ammonium sulfate"]
    CultureMech_007380 --> ing22_Ferrous_ammonium_sulfate
    ing23_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007380 --> ing23_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing23_Cupric_sulfate -.-> CHEBI_23414
    ing24_Sodium_selenite["Sodium selenite"]
    CultureMech_007380 --> ing24_Sodium_selenite
    CHEBI_48843["CHEBI:48843"]
    ing24_Sodium_selenite -.-> CHEBI_48843
    ing25_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007380 --> ing25_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing25_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing26_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007380 --> ing26_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing26_Potassium_dibasic_phosphate -.-> CHEBI_131527

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
2 Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
5.0 G_PER_L FOODON:03315426
yeast extract
 Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
3 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
4 Sodium sulfide
2Na.S 78.047 g/mol
2.073 MILLIMOLAR CHEBI:76208
sodium sulfide (anhydrous)
5 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
3.172 MILLIMOLAR CHEBI:91247
L-cysteine hydrochloride
6 2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
7.032e-06 MILLIMOLAR CHEBI:17905
coenzyme M
 Cross-references: KEGG:com
7 Sodium bicarbonate
CHO3.Na 84.006 g/mol
59.52 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
8 Potassium chloride
Cl.K 74.551 g/mol
4.493 MILLIMOLAR CHEBI:32588
potassium chloride
9 Resazurin
C12H7NO4 229.191 g/mol
0.0004363 MILLIMOLAR CHEBI:8806
Resazurin
10 Sodium chloride
Cl.Na 58.443 g/mol
376.0 MILLIMOLAR CHEBI:26710
sodium chloride
11 2-Methylpropanoate 0.05675 MILLIMOLAR
12 Zinc sulfate
O4S.Zn 161.453 g/mol
0.003478 MILLIMOLAR CHEBI:35176
zinc sulfate
13 Sodium tungstate
2Na.O4W 293.816 g/mol
0.003032 MILLIMOLAR CHEBI:63940
sodium tungstate
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.07848 MILLIMOLAR CHEBI:44557
nitrilotriacetic acid
15 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.005347 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
16 Nickel chloride
Cl2Ni 129.599 g/mol
0.001052 MILLIMOLAR CHEBI:34887
nickel dichloride
17 Ammonium chloride
Cl.H4N 53.492 g/mol
9.348 MILLIMOLAR CHEBI:31206
ammonium chloride
18 Magnesium sulfate
Mg.O4S 120.368 g/mol
14.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
19 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.004133 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
20 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
13.53 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
21 Cobalt chloride
2Cl.Co 129.839 g/mol
0.004203 MILLIMOLAR CHEBI:35696
cobalt dichloride
22 Pentanoate 0.04896 MILLIMOLAR
23 Ferrous ammonium sulfate 0.005355 MILLIMOLAR
24 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0004005 MILLIMOLAR CHEBI:23414
copper(II) sulfate
25 Sodium selenite
2Na.O3Se 172.937 g/mol
0.01156 MILLIMOLAR CHEBI:48843
disodium selenite
26 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
1.085 MILLIMOLAR CHEBI:3312
calcium dichloride
27 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
0.8038 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:47.016298Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 473, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:14.528452Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:48.529896Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007380
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:06.872813+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'McN Medium (Whitman" to "mcn_medium_whitman" for consistent matching
2026-06-05T07:41:13.442654+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:42.400101+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b8a0ff94d9d9b5c18a73ee93f8d4e11401efb735b9a32934091f1fd9a743bbfd
2026-08-15T20:09:28.304502+00:00 dedupe_identical_ingredients.py REMOVED_DUPLICATE_INGREDIENT_ROWS Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract.