Overview
- External ontology
- MEDIADB:474 (source link unavailable) — 'McNA Medium (Lupa
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/ Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_007381["mcna_medium_lupa"]
ing0_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_007381 --> ing0_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing0_Nitrilotriacetic_acid -.-> CHEBI_44557
ing1_Magnesium_chloride["Magnesium chloride"]
CultureMech_007381 --> ing1_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing1_Magnesium_chloride -.-> CHEBI_86345
ing2_Pentanoate["Pentanoate"]
CultureMech_007381 --> ing2_Pentanoate
ing3_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
CultureMech_007381 --> ing3_2_Mercaptoethanesulfonate
CHEBI_17905["CHEBI:17905"]
ing3_2_Mercaptoethanesulfonate -.-> CHEBI_17905
ing4_Sodium_bicarbonate["Sodium bicarbonate"]
CultureMech_007381 --> ing4_Sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing4_Sodium_bicarbonate -.-> CHEBI_32139
ing5_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007381 --> ing5_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing5_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing6_2_Methylpropanoate["2-Methylpropanoate"]
CultureMech_007381 --> ing6_2_Methylpropanoate
ing7_Resazurin["Resazurin"]
CultureMech_007381 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Tryptone["Tryptone"]
CultureMech_007381 --> ing8_Tryptone
ing9_Yeast_extract["Yeast extract"]
CultureMech_007381 --> ing9_Yeast_extract
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_007381 --> ing10_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007381 --> ing11_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing11_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing12_Sodium_sulfide["Sodium sulfide"]
CultureMech_007381 --> ing12_Sodium_sulfide
CHEBI_76208["CHEBI:76208"]
ing12_Sodium_sulfide -.-> CHEBI_76208
ing13_Ferrous_ammonium_sulfate["Ferrous ammonium sulfate"]
CultureMech_007381 --> ing13_Ferrous_ammonium_sulfate
ing14_Cysteine_HCl["Cysteine-HCl"]
CultureMech_007381 --> ing14_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing14_Cysteine_HCl -.-> CHEBI_91247
ing15_Cupric_sulfate["Cupric sulfate"]
CultureMech_007381 --> ing15_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing15_Cupric_sulfate -.-> CHEBI_23414
ing16_Nickel_chloride["Nickel chloride"]
CultureMech_007381 --> ing16_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing16_Nickel_chloride -.-> CHEBI_34887
ing17_Ammonium_chloride["Ammonium chloride"]
CultureMech_007381 --> ing17_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing17_Ammonium_chloride -.-> CHEBI_31206
ing18_Sodium_chloride["Sodium chloride"]
CultureMech_007381 --> ing18_Sodium_chloride
ing18_Sodium_chloride -.-> CHEBI_26710
ing19_Sodium_molybdate["Sodium molybdate"]
CultureMech_007381 --> ing19_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing19_Sodium_molybdate -.-> CHEBI_75215
ing20_Cobalt_chloride["Cobalt chloride"]
CultureMech_007381 --> ing20_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing20_Cobalt_chloride -.-> CHEBI_35696
ing21_Potassium_chloride["Potassium chloride"]
CultureMech_007381 --> ing21_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing21_Potassium_chloride -.-> CHEBI_32588
ing22_Acetate["Acetate"]
CultureMech_007381 --> ing22_Acetate
CHEBI_30089["CHEBI:30089"]
ing22_Acetate -.-> CHEBI_30089
ing23_Zinc_sulfate["Zinc sulfate"]
CultureMech_007381 --> ing23_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing23_Zinc_sulfate -.-> CHEBI_35176
ing24_Sodium_tungstate["Sodium tungstate"]
CultureMech_007381 --> ing24_Sodium_tungstate
CHEBI_63940["CHEBI:63940"]
ing24_Sodium_tungstate -.-> CHEBI_63940
ing25_Sodium_selenite["Sodium selenite"]
CultureMech_007381 --> ing25_Sodium_selenite
CHEBI_48843["CHEBI:48843"]
ing25_Sodium_selenite -.-> CHEBI_48843
ing26_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007381 --> ing26_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing26_Magnesium_sulfate -.-> CHEBI_32599
ing27_Manganese_sulfate["Manganese sulfate"]
CultureMech_007381 --> ing27_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing27_Manganese_sulfate -.-> CHEBI_86364
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.07848 MILLIMOLAR |
CHEBI:44557
nitrilotriacetic acid
|
|
| 2 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
13.53 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 | Pentanoate | 0.04896 MILLIMOLAR | ||
| 4 |
2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
|
7.032e-06 MILLIMOLAR |
CHEBI:17905
coenzyme M
|
Cross-references: KEGG:com |
| 5 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
59.52 MILLIMOLAR |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
1.085 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 7 | 2-Methylpropanoate | 0.05675 MILLIMOLAR | ||
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0004363 MILLIMOLAR |
CHEBI:8806
Resazurin
|
|
| 9 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 10 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 12 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
0.8038 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 13 |
Sodium sulfide
2Na.S 78.047 g/mol
|
2.073 MILLIMOLAR |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 14 | Ferrous ammonium sulfate | 0.005355 MILLIMOLAR | ||
| 15 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
3.172 MILLIMOLAR |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 16 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.0004005 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 17 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
0.001052 MILLIMOLAR |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
9.348 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 19 |
Sodium chloride
Cl.Na 58.443 g/mol
|
376.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 20 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.004133 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 21 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.004203 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 22 |
Potassium chloride
Cl.K 74.551 g/mol
|
4.493 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 23 |
Acetate
C2H3O2 59.044 g/mol
|
10.0 MILLIMOLAR |
CHEBI:30089
acetate
|
Cross-references: KEGG:ac |
| 24 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.003478 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 25 |
Sodium tungstate
2Na.O4W 293.816 g/mol
|
0.003032 MILLIMOLAR |
CHEBI:63940
sodium tungstate
|
|
| 26 |
Sodium selenite
2Na.O3Se 172.937 g/mol
|
0.01156 MILLIMOLAR |
CHEBI:48843
disodium selenite
|
|
| 27 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
14.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 28 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.005347 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mcna_medium_lupa (CultureMech:007381)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:47.025800Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 474, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:21.614601Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:48.541202Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007381 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:06.884009+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'McNA Medium (Lupa" to "mcna_medium_lupa" for consistent matching |
| 2026-06-05T07:41:13.458120+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:42.416931+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a60d24cd6e96bc76eaee16a17c6c413dc9fc190bf96019830e97d66d83fb575b |
| 2026-08-15T20:09:28.320953+00:00 | dedupe_identical_ingredients.py | REMOVED_DUPLICATE_INGREDIENT_ROWS | Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract. |