ams1_pyruvate_glycine

CultureMech:007382 bacterial DEFINED LIQUID
Original name: 'AMS1 (Pyruvate+Glycine

Overview

External ontology
MEDIADB:475 (source link unavailable) — 'AMS1 (Pyruvate+Glycine
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007382["ams1_pyruvate_glycine"]
    ing0_Pyruvate["Pyruvate"]
    CultureMech_007382 --> ing0_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing0_Pyruvate -.-> CHEBI_15361
    ing1_Biotin["Biotin"]
    CultureMech_007382 --> ing1_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing1_Biotin -.-> CHEBI_15956
    ing2_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_007382 --> ing2_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing2_Sodium_bicarbonate -.-> CHEBI_32139
    ing3_4_Amino_5_hydroxymethyl_2_methylpyrimidine["4-Amino-5-hydroxymethyl-2-methylpyrimidine"]
    CultureMech_007382 --> ing3_4_Amino_5_hydroxymethyl_2_methylpyrimidine
    ing4_Glycine["Glycine"]
    CultureMech_007382 --> ing4_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing4_Glycine -.-> CHEBI_15428
    ing5_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007382 --> ing5_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing5_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing6_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007382 --> ing6_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing6_Magnesium_chloride -.-> CHEBI_86345
    ing7_Nickel_chloride["Nickel chloride"]
    CultureMech_007382 --> ing7_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing7_Nickel_chloride -.-> CHEBI_34887
    ing8_Sodium_chloride["Sodium chloride"]
    CultureMech_007382 --> ing8_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing8_Sodium_chloride -.-> CHEBI_26710
    ing9_Potassium_chloride["Potassium chloride"]
    CultureMech_007382 --> ing9_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing9_Potassium_chloride -.-> CHEBI_32588
    ing10__Iron_III["'Iron(III"]
    CultureMech_007382 --> ing10__Iron_III
    ing11_Sodium_selenite["Sodium selenite"]
    CultureMech_007382 --> ing11_Sodium_selenite
    CHEBI_48843["CHEBI:48843"]
    ing11_Sodium_selenite -.-> CHEBI_48843
    ing12_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007382 --> ing12_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing12_Ammonium_sulfate -.-> CHEBI_62946
    ing13_Manganese_chloride["Manganese chloride"]
    CultureMech_007382 --> ing13_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing13_Manganese_chloride -.-> CHEBI_63041
    ing14_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007382 --> ing14_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing14_Sodium_molybdate -.-> CHEBI_75215
    ing15_DL_methionine["DL-methionine"]
    CultureMech_007382 --> ing15_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing15_DL_methionine -.-> CHEBI_16811
    ing16_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007382 --> ing16_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing16_Zinc_sulfate -.-> CHEBI_35176
    ing17_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
    CultureMech_007382 --> ing17_Sodium_dihydrogen_phosphate
    CHEBI_37585["CHEBI:37585"]
    ing17_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
    ing18_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007382 --> ing18_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing18_Magnesium_sulfate -.-> CHEBI_32599
    ing19_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007382 --> ing19_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing19_Cobalt_chloride -.-> CHEBI_35696

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Pyruvate
C3H3O3 87.054 g/mol
1.0 MILLIMOLAR CHEBI:15361
pyruvate
 Cross-references: KEGG:pyr
2 Biotin
C10H16N2O3S 244.316 g/mol
4e-06 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
3 Sodium bicarbonate
CHO3.Na 84.006 g/mol
6.0 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
4 4-Amino-5-hydroxymethyl-2-methylpyrimidine 1e-05 MILLIMOLAR Cross-references: KEGG:4ahmmp
5 Glycine
C2H5NO2 75.067 g/mol
0.05 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
6 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
10.0 MILLIMOLAR CHEBI:3312
calcium dichloride
7 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
27.0 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
8 Nickel chloride
Cl2Ni 129.599 g/mol
1e-06 MILLIMOLAR CHEBI:34887
nickel dichloride
9 Sodium chloride
Cl.Na 58.443 g/mol
481.0 MILLIMOLAR CHEBI:26710
sodium chloride
10 Potassium chloride
Cl.K 74.551 g/mol
9.0 MILLIMOLAR CHEBI:32588
potassium chloride
11 'Iron(III 0.000117 MILLIMOLAR
12 Sodium selenite
2Na.O3Se 172.937 g/mol
1e-06 MILLIMOLAR CHEBI:48843
disodium selenite
13 Ammonium sulfate
2H4N.O4S 132.141 g/mol
0.4 MILLIMOLAR CHEBI:62946
ammonium sulfate
14 Manganese chloride
Cl2Mn 125.844 g/mol
9e-06 MILLIMOLAR CHEBI:63041
manganese(II) chloride
15 Sodium molybdate
MoO4.2Na 205.916 g/mol
3e-07 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
16 DL-methionine
C5H11NO2S 149.215 g/mol
0.05 MILLIMOLAR CHEBI:16811
methionine
17 Zinc sulfate
O4S.Zn 161.453 g/mol
8e-07 MILLIMOLAR CHEBI:35176
zinc sulfate
18 Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
0.05 MILLIMOLAR CHEBI:37585
sodium dihydrogenphosphate
19 Magnesium sulfate
Mg.O4S 120.368 g/mol
28.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
20 Cobalt chloride
2Cl.Co 129.839 g/mol
5e-07 MILLIMOLAR CHEBI:35696
cobalt dichloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:47.035033Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 475, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:33.473703Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:48.551101Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007382
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:06.894084+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'AMS1 (Pyruvate+Glycine" to "ams1_pyruvate_glycine" for consistent matching
2026-06-05T07:40:48.643583+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-09T01:42:18.116828+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-08-06T03:05:23.168468+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 08b97e38407a08f0e937158165e558daecaf4b92cd88e8827ca00951f08a8dd8