ams1_pyruvate_glycine

CultureMech:007389 bacterial DEFINED LIQUID
Original name: 'AMS1 (Pyruvate+Glycine

Overview

External ontology
MEDIADB:481 (source link unavailable) — 'AMS1 (Pyruvate+Glycine
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007389["ams1_pyruvate_glycine"]
    ing0_Thiamine["Thiamine"]
    CultureMech_007389 --> ing0_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing0_Thiamine -.-> CHEBI_18385
    ing1_Pyruvate["Pyruvate"]
    CultureMech_007389 --> ing1_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing1_Pyruvate -.-> CHEBI_15361
    ing2_Biotin["Biotin"]
    CultureMech_007389 --> ing2_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing2_Biotin -.-> CHEBI_15956
    ing3_Nickel_chloride["Nickel chloride"]
    CultureMech_007389 --> ing3_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing3_Nickel_chloride -.-> CHEBI_34887
    ing4_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007389 --> ing4_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing4_Magnesium_chloride -.-> CHEBI_86345
    ing5_Glycine["Glycine"]
    CultureMech_007389 --> ing5_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing5_Glycine -.-> CHEBI_15428
    ing6_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_007389 --> ing6_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing6_Sodium_bicarbonate -.-> CHEBI_32139
    ing7_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007389 --> ing7_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing7_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing8_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007389 --> ing8_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing8_Sodium_molybdate -.-> CHEBI_75215
    ing9_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007389 --> ing9_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing9_Magnesium_sulfate -.-> CHEBI_32599
    ing10_D_Ribose_5_phosphate["D-Ribose 5-phosphate"]
    CultureMech_007389 --> ing10_D_Ribose_5_phosphate
    ing11__Iron_III["'Iron(III"]
    CultureMech_007389 --> ing11__Iron_III
    ing12_Potassium_chloride["Potassium chloride"]
    CultureMech_007389 --> ing12_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing12_Potassium_chloride -.-> CHEBI_32588
    ing13_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007389 --> ing13_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing13_Cobalt_chloride -.-> CHEBI_35696
    ing14_Sodium_chloride["Sodium chloride"]
    CultureMech_007389 --> ing14_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing14_Sodium_chloride -.-> CHEBI_26710
    ing15_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007389 --> ing15_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing15_Ammonium_sulfate -.-> CHEBI_62946
    ing16_Manganese_chloride["Manganese chloride"]
    CultureMech_007389 --> ing16_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing16_Manganese_chloride -.-> CHEBI_63041
    ing17_Sodium_selenite["Sodium selenite"]
    CultureMech_007389 --> ing17_Sodium_selenite
    CHEBI_48843["CHEBI:48843"]
    ing17_Sodium_selenite -.-> CHEBI_48843
    ing18_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007389 --> ing18_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing18_Zinc_sulfate -.-> CHEBI_35176
    ing19_DL_methionine["DL-methionine"]
    CultureMech_007389 --> ing19_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing19_DL_methionine -.-> CHEBI_16811

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Thiamine
C12H17N4OS 265.362 g/mol
0.06 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
2 Pyruvate
C3H3O3 87.054 g/mol
1.0 MILLIMOLAR CHEBI:15361
pyruvate
 Cross-references: KEGG:pyr
3 Biotin
C10H16N2O3S 244.316 g/mol
4e-06 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
4 Nickel chloride
Cl2Ni 129.599 g/mol
1e-06 MILLIMOLAR CHEBI:34887
nickel dichloride
5 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
27.0 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
6 Glycine
C2H5NO2 75.067 g/mol
0.05 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
7 Sodium bicarbonate
CHO3.Na 84.006 g/mol
6.0 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
8 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
10.0 MILLIMOLAR CHEBI:3312
calcium dichloride
9 Sodium molybdate
MoO4.2Na 205.916 g/mol
3e-07 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
10 Magnesium sulfate
Mg.O4S 120.368 g/mol
28.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
11 D-Ribose 5-phosphate 0.001 MILLIMOLAR Cross-references: KEGG:r5p
12 'Iron(III 0.000117 MILLIMOLAR
13 Potassium chloride
Cl.K 74.551 g/mol
9.0 MILLIMOLAR CHEBI:32588
potassium chloride
14 Cobalt chloride
2Cl.Co 129.839 g/mol
5e-07 MILLIMOLAR CHEBI:35696
cobalt dichloride
15 Sodium chloride
Cl.Na 58.443 g/mol
481.0 MILLIMOLAR CHEBI:26710
sodium chloride
16 Ammonium sulfate
2H4N.O4S 132.141 g/mol
0.4 MILLIMOLAR CHEBI:62946
ammonium sulfate
17 Manganese chloride
Cl2Mn 125.844 g/mol
9e-06 MILLIMOLAR CHEBI:63041
manganese(II) chloride
18 Sodium selenite
2Na.O3Se 172.937 g/mol
1e-06 MILLIMOLAR CHEBI:48843
disodium selenite
19 Zinc sulfate
O4S.Zn 161.453 g/mol
8e-07 MILLIMOLAR CHEBI:35176
zinc sulfate
20 DL-methionine
C5H11NO2S 149.215 g/mol
0.05 MILLIMOLAR CHEBI:16811
methionine

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T07:21:47.088022Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 481, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:09.507892Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:48.620221Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007389
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:06.966870+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'AMS1 (Pyruvate+Glycine" to "ams1_pyruvate_glycine" for consistent matching
2026-06-05T07:40:29.503286+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:04.558837+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b92de7288327a96748a51a5ed2b8e22b55da3bd1c62a52ff787e175531b5be97