modified_m63_medium_with_glucose_and_2_5_m_nacl (CultureMech:007428)

Overview

External ontology

MEDIADB:7 (source link unavailable) — Modified M63 medium with glucose and 2.5 M NaCl

Applications

Cultivation of genome-sequenced organisms
Metabolic modeling
Systems biology research

Synonyms

{'name': 'modified_m63_medium_with_glucose_and_0_6_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:4', 'original_category': 'bacterial'}
{'name': 'modified_m63_medium_with_glucose_and_0_75_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:5', 'original_category': 'bacterial'}
{'name': 'modified_m63_medium_with_glucose_and_1_5_m_nacl', 'source': 'unknown', 'source_id': 'MEDIADB:6', 'original_category': 'bacterial'}

High-metal flag

True

Notes

Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007428["modified_m63_medium_with_glucose_and_2_5_m_nacl"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007428 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Potassium_hydroxide["Potassium hydroxide"]
    CultureMech_007428 --> ing1_Potassium_hydroxide
    CHEBI_32035["CHEBI:32035"]
    ing1_Potassium_hydroxide -.-> CHEBI_32035
    ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007428 --> ing2_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007428 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007428 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007428 --> ing5_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing5_Ferrous_sulfate -.-> CHEBI_75832
    ing6_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007428 --> ing6_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing6_Ammonium_sulfate -.-> CHEBI_62946

Ingredients (7)

#	Ingredient	Concentration	Ontology	Notes
1	D-Glucose C6H12O6 180.156 g/mol	20.0 MILLIMOLAR	CHEBI:17634 D-glucose	Cross-references: KEGG:glc_D
2	Potassium hydroxide HO.K 56.105 g/mol	75.0 MILLIMOLAR	CHEBI:32035 potassium hydroxide
3	Potassium dibasic phosphate HO4P.2K 174.174 g/mol	100.0 MILLIMOLAR	CHEBI:131527 dipotassium hydrogen phosphate
4	Sodium chloride Cl.Na 58.443 g/mol	600.0 MILLIMOLAR	CHEBI:26710 sodium chloride
5	Magnesium sulfate Mg.O4S 120.368 g/mol	0.16 MILLIMOLAR	CHEBI:32599 magnesium sulfate
6	Ferrous sulfate Fe.O4S 151.908 g/mol	0.0018 MILLIMOLAR	CHEBI:75832 iron(2+) sulfate (anhydrous)
7	Ammonium sulfate 2H4N.O4S 132.141 g/mol	15.0 MILLIMOLAR	CHEBI:62946 ammonium sulfate

Preparation

Dissolve all ingredients in distilled water to specified concentrations
Adjust pH if specified in original formulation
Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

modified_m63_medium_with_glucose_and_0_6_m_nacl (CultureMech:007395)
modified_m63_medium_with_glucose_and_0_75_m_nacl (CultureMech:007406)
modified_m63_medium_with_glucose_and_1_5_m_nacl (CultureMech:007417)
modified_m63_medium_with_glucose_and_2_5_m_nacl (CultureMech:007428)

Timestamp	Curator	Action	Notes
2026-01-27T07:21:42.551660Z	mediadb-import	Imported from MediaDB	Source: MediaDB (Institute for Systems Biology), Medium ID: 7, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:49.005766Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007428
2026-03-17T06:30:02.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.384876+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified M63 medium with glucose and 2.5 M NaCl" to "modified_m63_medium_with_glucose_and_2_5_m_nacl" for consistent matching
2026-06-05T07:41:36.959693+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:48.664324+00:00	recipe-merger	Merged 4 duplicate recipes into canonical record	Sources: modified_m63_medium_with_glucose_and_0_6_m_nacl, modified_m63_medium_with_glucose_and_0_75_m_nacl, modified_m63_medium_with_glucose_and_1_5_m_nacl, modified_m63_medium_with_glucose_and_2_5_m_nacl
2026-08-06T03:05:48.664373+00:00	merge_recipes.py	MERGED_RECIPES	merged 4 source recipe(s) on fingerprint 7f96d449859a85c3cd5a5de055cd4b2bd14d1ada64de73dddc1e6bd4c0726175