Overview
- External ontology
- MEDIADB:84 (source link unavailable) — Pjc with 3,4-dhpa
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007431["pjc_with_3_4_dhpa"]
ing0_3_4_Dihydroxyphenylacetate["3,4-Dihydroxyphenylacetate"]
CultureMech_007431 --> ing0_3_4_Dihydroxyphenylacetate
ing1_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007431 --> ing1_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing1_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing2_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_007431 --> ing2_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing2_Nitrilotriacetic_acid -.-> CHEBI_44557
ing3_Cobaltous_sulfate["Cobaltous sulfate"]
CultureMech_007431 --> ing3_Cobaltous_sulfate
CHEBI_53470["CHEBI:53470"]
ing3_Cobaltous_sulfate -.-> CHEBI_53470
ing4_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007431 --> ing4_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing4_Magnesium_sulfate -.-> CHEBI_32599
ing5_Manganese_sulfate["Manganese sulfate"]
CultureMech_007431 --> ing5_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing5_Manganese_sulfate -.-> CHEBI_86364
ing6_Ammonium_chloride["Ammonium chloride"]
CultureMech_007431 --> ing6_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing6_Ammonium_chloride -.-> CHEBI_31206
ing7_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007431 --> ing7_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing7_Ferrous_sulfate -.-> CHEBI_75832
ing8_Zinc_sulfate["Zinc sulfate"]
CultureMech_007431 --> ing8_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing8_Zinc_sulfate -.-> CHEBI_35176
ing9_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
CultureMech_007431 --> ing9_Sodium_dihydrogen_phosphate
CHEBI_37585["CHEBI:37585"]
ing9_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 3,4-Dihydroxyphenylacetate | 5.0 MILLIMOLAR | Cross-references: KEGG:34dhpha | |
| 2 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
18.6 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.5 MILLIMOLAR |
CHEBI:44557
nitrilotriacetic acid
|
|
| 4 |
Cobaltous sulfate
Co.O4S 154.996 g/mol
|
0.004 MILLIMOLAR |
CHEBI:53470
cobalt(2+) sulfate
|
|
| 5 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.811 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 6 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.017 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 7 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
37.4 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 8 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.043 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 9 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.01 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 10 |
Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
|
7.25 MILLIMOLAR |
CHEBI:37585
sodium dihydrogenphosphate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pjc_with_3_4_dhpa (CultureMech:007431)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.360978Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 84, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:49.021067Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007431 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.401076+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pjc with 3,4-dhpa" to "pjc_with_3_4_dhpa" for consistent matching |
| 2026-06-05T07:41:42.072258+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-09T01:42:25.346503+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:53.076625+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 42973c4cca1610aa0ca579a4693ad6346dab8c455094d17299e3ac2b22024254 |