Overview
- External ontology
- MEDIADB:89 (source link unavailable) — P2, no butanol challenge
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007436["p2_no_butanol_challenge"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007436 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Biotin["Biotin"]
CultureMech_007436 --> ing1_Biotin
CHEBI_15956["CHEBI:15956"]
ing1_Biotin -.-> CHEBI_15956
ing2_Thiamine["Thiamine"]
CultureMech_007436 --> ing2_Thiamine
CHEBI_18385["CHEBI:18385"]
ing2_Thiamine -.-> CHEBI_18385
ing3_4_Aminobenzoate["4-Aminobenzoate"]
CultureMech_007436 --> ing3_4_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing3_4_Aminobenzoate -.-> CHEBI_17836
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007436 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_007436 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007436 --> ing6_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing7_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007436 --> ing7_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing7_Magnesium_sulfate -.-> CHEBI_32599
ing8_Manganese_sulfate["Manganese sulfate"]
CultureMech_007436 --> ing8_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing8_Manganese_sulfate -.-> CHEBI_86364
ing9_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007436 --> ing9_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing9_Ferrous_sulfate -.-> CHEBI_75832
ing10_Ammonium_Acetate["Ammonium Acetate"]
CultureMech_007436 --> ing10_Ammonium_Acetate
CHEBI_62947["CHEBI:62947"]
ing10_Ammonium_Acetate -.-> CHEBI_62947
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
123.21 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Biotin
C10H16N2O3S 244.316 g/mol
|
4.5429e-05 MILLIMOLAR |
CHEBI:15956
biotin
|
Cross-references: KEGG:btn |
| 3 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.00368963 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 4 |
4-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.00809302 MILLIMOLAR |
CHEBI:17836
4-aminobenzoate
|
Cross-references: KEGG:4abz |
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
3.18564 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
|
0.189918 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 7 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
4.07785 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.900619 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 9 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.0656655 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 10 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0399165 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 11 |
Ammonium Acetate
C2H3O2.H4N 77.083 g/mol
|
31.6775 MILLIMOLAR |
CHEBI:62947
ammonium acetate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- p2_no_butanol_challenge (CultureMech:007436)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.404278Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 89, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:49.051765Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007436 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.435111+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "P2, no butanol challenge" to "p2_no_butanol_challenge" for consistent matching |
| 2026-06-05T07:41:40.746921+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:52.153312+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 91f3671be2e9393d8427307d148aeeba1bb2422061b2e90fa449133ef58262e6 |