Overview
- External ontology
- TOGO:M1002 — Neutral Oligotrophic Haloarchaeal Medium NO.3
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1002 Original source: JCM - JCM_M954-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=954
Composition
flowchart LR
CultureMech_007515["neutral_oligotrophic_haloarchaeal_medium_no_3"]
ing0_Distilled_water["Distilled water"]
CultureMech_007515 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007515 --> ing1_Yeast_extract
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_007515 --> ing2_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Sodium_acetate -.-> CHEBI_32954
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_007515 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_007515 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_Fish_peptone__Fluka_["Fish peptone (Fluka)"]
CultureMech_007515 --> ing5_Fish_peptone__Fluka_
ing6_agar["agar"]
CultureMech_007515 --> ing6_agar
CHEBI_2509["CHEBI:2509"]
ing6_agar -.-> CHEBI_2509
ing7_Sodium_formate["Sodium formate"]
CultureMech_007515 --> ing7_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing7_Sodium_formate -.-> CHEBI_62965
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
233 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.25 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.25 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.25 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 6 | Fish peptone (Fluka) | 0.25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 | agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.25 G_PER_L |
CHEBI:62965
sodium formate
|
Properties: Defined component, Inorganic compound, Simple component |
Solutions
MDS salt water (see Medium [M578])
Dose: 767 G_PER_L
Cross-reference to Medium M578
1 M NH4Cl solution*
Dose: 5 G_PER_L
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Solution
Potassium phosphate buffer (see Medium [M578])
Dose: 2 G_PER_L
Cross-reference to Medium M578
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1002_Neutral_Oligotrophic_Haloarchaeal_Medium_NO.3 (CultureMech:007515)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.736805Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1002 |
| 2026-01-28T19:48:14.991783Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:24.917318+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:49.326452Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007515 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.655350+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Neutral Oligotrophic Haloarchaeal Medium NO.3" to "neutral_oligotrophic_haloarchaeal_medium_no_3" for consistent matching |
| 2026-05-16T09:03:06.797672+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:29.593631+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:24.387515+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 33669e3466b54ee20e86c540fca227e130be82ba94f8255796580a8351869a68 |