Overview
- External ontology
- TOGO:M1015 — G-S Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1015 Original source: JCM - JCM_M965 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=965
Composition
flowchart LR
CultureMech_007529["g_s_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007529 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_007529 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_007529 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Resazurin["Resazurin"]
CultureMech_007529 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Cellobiose["Cellobiose"]
CultureMech_007529 --> ing4_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing4_Cellobiose -.-> CHEBI_17057
ing5_MOPS["MOPS"]
CultureMech_007529 --> ing5_MOPS
CHEBI_39074["CHEBI:39074"]
ing5_MOPS -.-> CHEBI_39074
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007529 --> ing6_Yeast_extract__BD_Difco_
ing7_Urea["Urea"]
CultureMech_007529 --> ing7_Urea
CHEBI_16199["CHEBI:16199"]
ing7_Urea -.-> CHEBI_16199
ing8_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_007529 --> ing8_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007529 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_007529 --> ing10_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing10_Nitrogen_gas -.-> CHEBI_17997
ing11_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007529 --> ing11_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_2H2O -.-> CHEBI_86158
ing12_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007529 --> ing12_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing12_MgCl2_6H2O -.-> CHEBI_86345
ing13_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_007529 --> ing13_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_7H2O -.-> CHEBI_75836
ing14_ZnCl2["ZnCl2"]
CultureMech_007529 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
11.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Defined component, Inorganic compound [Merged 2 duplicates: 1.0, 10.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 5 |
Cellobiose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 6 | MOPS | 20.9 G_PER_L |
CHEBI:39074
MOPS
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 7 | Yeast extract (BD-Difco) | 4.5 G_PER_L | Properties: Undefined component | |
| 8 |
Urea
CH4N2O 60.056 g/mol
|
2.9 G_PER_L |
CHEBI:16199
urea
|
Properties: Simple component, Defined component, Organic compound |
| 9 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
3.03 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component [Merged 2 duplicates: 3.0, 0.03] |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.375 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 14 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.031 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
10 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
Solutions
Mineral solution
Dose: 1 G_PER_L
See mediadive_5281_Mineral_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1015_G-S_Medium (CultureMech:007529)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.760673Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1015 |
| 2026-01-28T19:48:14.030991Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:23.249455+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:51.489472Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:49.430754Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007529 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.769056+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "G-S Medium" to "g_s_medium" for consistent matching |
| 2026-05-16T09:03:06.893625+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:29.700890+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.699082+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:04.866815+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8886a6d80fc29a3bcf6ac29e7e2a3e96cd71c35263675187af43ad4718c59f23 |