Overview
- External ontology
- TOGO:M1045 — Diluted Nitrate Mineral Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1045 Original source: JCM - JCM_M992 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=992
Composition
flowchart LR
CultureMech_007561["diluted_nitrate_mineral_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007561 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007561 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007561 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KNO3["KNO3"]
CultureMech_007561 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_methane["methane"]
CultureMech_007561 --> ing4_methane
ing5_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_007561 --> ing5_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_7H2O -.-> CHEBI_75836
ing6_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_007561 --> ing6_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing6_CuSO4_5H2O -.-> CHEBI_31440
ing7_EDTA_2Na["EDTA・2Na"]
CultureMech_007561 --> ing7_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing7_EDTA_2Na -.-> CHEBI_64734
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1890.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 990.0, 900.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.03 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
0.2 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 | methane | variable VARIABLE | Properties: Gas | |
| 6 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.25 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Modified SL-4 trace element solution (see below)
Dose: 1 G_PER_L
Chelated iron solution (see Medium [M863])
Dose: 10 G_PER_L
Cross-reference to Medium M863
0.2 M KH2PO4 solution
Dose: 5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
0.2 M Na2HPO4 solution
Dose: 5 G_PER_L
Role: Buffer; Properties: Defined component, Solution, Inorganic compound
SL-6 trace element solution (see Medium [M158])
Dose: 100 G_PER_L
Cross-reference to Medium M158
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1045_Diluted_Nitrate_Mineral_Medium (CultureMech:007561)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.818996Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1045 |
| 2026-01-28T19:48:38.474237Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: methane |
| 2026-03-09T08:05:04.374788+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:36.328518Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:49.681423Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007561 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.030680+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Diluted Nitrate Mineral Medium" to "diluted_nitrate_mineral_medium" for consistent matching |
| 2026-05-16T09:03:07.157972+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:29.979506+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:20.878303+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:05.188839+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fa8fa1862f1b4042ed2cfa42bbe5c85883514890296d6e1f2a2d666f5ad584a1 |