Overview
- External ontology
- TOGO:M1048 — MBM Medium 2
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1048 Original source: JCM - JCM_M994 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=994
Composition
flowchart LR
CultureMech_007564["mbm_medium_2"]
ing0_Distilled_water["Distilled water"]
CultureMech_007564 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007564 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007564 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007564 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_007564 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_007564 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_007564 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_KCl["KCl"]
CultureMech_007564 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Sodium_acetate_3H2O["Sodium acetate・3H2O"]
CultureMech_007564 --> ing8_Sodium_acetate_3H2O
CHEBI_32954["CHEBI:32954"]
ing8_Sodium_acetate_3H2O -.-> CHEBI_32954
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007564 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_007564 --> ing10_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing10_Nitrogen_gas -.-> CHEBI_17997
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
80 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 8 |
KCl
Cl.K 74.551 g/mol
|
1.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
Sodium acetate・3H2O
C2H3O2.Na 82.034 g/mol
|
1.6 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
8% NaHCO3 solution*
Dose: 50 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1 M Disodium fumarate solution
Dose: 40 G_PER_L
Properties: Defined component, Solution, Organic compound
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
5% Na2S・9H2O solution
Dose: 6 G_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
5% L--Cysteine・HCl・H2O solution
Dose: 6 G_PER_L
Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1048_MBM_Medium_2 (CultureMech:007564)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.824193Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1048 |
| 2026-01-28T19:48:20.686003Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:34.293066+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:03.848811Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:49.705970Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007564 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.055701+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MBM Medium 2" to "mbm_medium_2" for consistent matching |
| 2026-05-16T09:03:07.175206+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:29.998324+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:20.897700+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:46.788896+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:05.210480+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1096b6b6f61313a57f55fdb4073fabd42ff915d934d09ec3655b30789067a53d |