Overview
- External ontology
- TOGO:M104 — CM3 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M104 Original source: JCM - JCM_M112 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=112
Composition
flowchart LR
CultureMech_007566["cm3_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007566 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007566 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007566 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_K2HPO4["K2HPO4"]
CultureMech_007566 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_007566 --> ing4_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_7H2O -.-> CHEBI_75836
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_007566 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_MOPS["MOPS"]
CultureMech_007566 --> ing6_MOPS
CHEBI_39074["CHEBI:39074"]
ing6_MOPS -.-> CHEBI_39074
ing7_Cellobiose_or_Cellulose_MN_300["Cellobiose or Cellulose MN 300"]
CultureMech_007566 --> ing7_Cellobiose_or_Cellulose_MN_300
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007566 --> ing8_Yeast_extract__BD_Difco_
ing9_Urea["Urea"]
CultureMech_007566 --> ing9_Urea
CHEBI_16199["CHEBI:16199"]
ing9_Urea -.-> CHEBI_16199
ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_007566 --> ing10_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L__Cysteine_HCl_H2O -.-> CHEBI_91248
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
4.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 7 | MOPS | 20 G_PER_L |
CHEBI:39074
MOPS
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 8 | Cellobiose or Cellulose MN 300 | 10 G_PER_L | Role: Carbon source; Properties: Simple component, Organic compound, Defined component | |
| 9 | Yeast extract (BD-Difco) | 6 G_PER_L | Properties: Undefined component | |
| 10 |
Urea
CH4N2O 60.056 g/mol
|
1.5 G_PER_L |
CHEBI:16199
urea
|
Properties: Simple component, Defined component, Organic compound |
| 11 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M104_CM3_Medium (CultureMech:007566)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.807091Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M104 |
| 2026-01-28T19:48:22.778224Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:49.722885Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007566 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.073586+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CM3 Medium" to "cm3_medium" for consistent matching |
| 2026-06-05T07:40:30.018994+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:20.913751+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:46.793822+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:05.234216+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 101b5a16aa1267a4364adc097b77839568f290cb3ad723599f84d4e64030c54d |