Overview
- External ontology
- TOGO:M1063 — Halophilic SOB Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1063 Original source: JCM - JCM_M1006 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1006
Composition
flowchart LR
CultureMech_007580["halophilic_sob_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007580 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007580 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007580 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007580 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_007580 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_007580 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_HCl__32__["HCl (32%)"]
CultureMech_007580 --> ing6_HCl__32__
ing7_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007580 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_H3BO3["H3BO3"]
CultureMech_007580 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007580 --> ing9_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_4H2O -.-> CHEBI_86368
ing10_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_007580 --> ing10_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing10_CoCl2_6H2O -.-> CHEBI_35696
ing11_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_007580 --> ing11_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_6H2O -.-> CHEBI_34887
ing12_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_007580 --> ing12_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_2H2O -.-> CHEBI_86318
ing13_ZnCl2["ZnCl2"]
CultureMech_007580 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_007580 --> ing14_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing14_Na2SeO3_5H2O -.-> CHEBI_131361
ing15_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_007580 --> ing15_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing15_Na2WO4_2H2O -.-> CHEBI_63939
ing16_AlCl3["AlCl3"]
CultureMech_007580 --> ing16_AlCl3
CHEBI_30114["CHEBI:30114"]
ing16_AlCl3 -.-> CHEBI_30114
ing17_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_007580 --> ing17_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing17_FeCl2_4H2O -.-> CHEBI_86249
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.6 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | HCl (32%) | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 8 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.05 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.05 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.05 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.03 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.05 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.05 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.05 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
AlCl3
AlCl3 133.341 g/mol
|
0.05 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
1 M NaHCO3 solution*
Dose: 10 G_PER_L
Role: Buffer; Properties: Solution, Inorganic compound, Defined component
1 M NH4Cl solution
Dose: 5 G_PER_L
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Solution
1 M K2HPO4 solution
Dose: 3 G_PER_L
Role: Buffer; Properties: Inorganic compound, Solution, Defined component
1 M Na2S2O3 solution
Dose: 20 G_PER_L
Properties: Defined component, Inorganic compound, Solution
0.5 M EDTA solution
Dose: 1 G_PER_L
Properties: Defined component, Solution, Organic compound
Trace metal solution* (see below)
Dose: 0.06 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 0.06 G_PER_L
Cross-reference to Medium M190
Vitamin B12 solution (see Medium [M401])
Dose: 6 G_PER_L
Cross-reference to Medium M401
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- halophilic_sob_medium (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.852346Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1063 |
| 2026-01-28T19:48:41.757148Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:10.425940+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:09:42.204514Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:49.826773Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007580 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.178039+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halophilic SOB Medium" to "halophilic_sob_medium" for consistent matching |
| 2026-05-16T09:03:36.873493+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:08.057424+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:48.497426+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:59.613559+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.359961+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 78b818a0ac12c333eb096e72dc47077f600c6b14bf44c1e335017a7e80e014b2 |