Overview
- External ontology
- TOGO:M1072 — NASU Iron Reducer Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1072 Original source: JCM - JCM_M1014 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1014
Composition
flowchart LR
CultureMech_007590["nasu_iron_reducer_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007590 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007590 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_007590 --> ing2_Yeast_extract
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007590 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_007590 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_007590 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007590 --> ing6_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing6_Carbon_dioxide_gas -.-> CHEBI_16526
ing7_Nitrogen_gas["Nitrogen gas"]
CultureMech_007590 --> ing7_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing7_Nitrogen_gas -.-> CHEBI_17997
ing8_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007590 --> ing8_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_2H2O -.-> CHEBI_75213
ing9_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007590 --> ing9_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_4H2O -.-> CHEBI_86368
ing10_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_007590 --> ing10_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_7H2O -.-> CHEBI_32312
ing11_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_007590 --> ing11_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_2H2O -.-> CHEBI_86318
ing12_CoSO4_7H2O["CoSO4・7H2O"]
CultureMech_007590 --> ing12_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing12_CoSO4_7H2O -.-> CHEBI_91244
ing13_VOSO4_xH2O["VOSO4・xH2O"]
CultureMech_007590 --> ing13_VOSO4_xH2O
ing14_Na2B4O7_10H2O["Na2B4O7・10H2O"]
CultureMech_007590 --> ing14_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing14_Na2B4O7_10H2O -.-> CHEBI_131366
ing15_iron_III__citrate["iron(III) citrate"]
CultureMech_007590 --> ing15_iron_III__citrate
CHEBI_144421["CHEBI:144421"]
ing15_iron_III__citrate -.-> CHEBI_144421
ing16_NaOH["NaOH"]
CultureMech_007590 --> ing16_NaOH
CHEBI_32145["CHEBI:32145"]
ing16_NaOH -.-> CHEBI_32145
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1001.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 900.0, 1.0, 100.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 8 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 9 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoSO4・7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.01 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | VOSO4・xH2O | 0.03 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 15 |
Na2B4O7・10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
4.5 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
iron(III) citrate
C6H5FeO7 244.944 g/mol
|
2.4 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 17 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
Modified Allen's trace metal solution (see below)
Dose: 1 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Iron(III) citrate solution (see below)
Dose: 100 G_PER_L
130 mM FeCl2 solution
Dose: 10 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
25 mM L--Cysteine・HCI solution
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1072_NASU_Iron_Reducer_Medium (CultureMech:007590)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.869803Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1072 |
| 2026-01-28T19:48:16.088915Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:26.558435+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:08:55.167766Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:49.900002Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007590 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.252636+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NASU Iron Reducer Medium" to "nasu_iron_reducer_medium" for consistent matching |
| 2026-05-16T09:03:07.360743+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.203851+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:21.056525+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:46.846659+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:05.499403+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9a6125e81dec813a5d702d1e66c7a1c885aced5cead5f29f0691317cc631a5c4 |