Overview
- External ontology
- TOGO:M1082 — MM4Y Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1082 Original source: JCM - JCM_M1021 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1021
Composition
flowchart LR
CultureMech_007598["mm4y_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007598 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Agar__Purified_["Agar (Purified)"]
CultureMech_007598 --> ing1_Agar__Purified_
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007598 --> ing2_Yeast_extract__BD_Difco_
ing3_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007598 --> ing3_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_7H2O -.-> CHEBI_31795
ing4_NaCl["NaCl"]
CultureMech_007598 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007598 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_007598 --> ing6_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_7H2O -.-> CHEBI_75836
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_007598 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_KH2PO4["KH2PO4"]
CultureMech_007598 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_Na2HPO4_12H2O["Na2HPO4・12H2O"]
CultureMech_007598 --> ing9_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing9_Na2HPO4_12H2O -.-> CHEBI_34683
ing10_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007598 --> ing10_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_2H2O -.-> CHEBI_75213
ing11_H3BO3["H3BO3"]
CultureMech_007598 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_007598 --> ing12_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing12_CoCl2_6H2O -.-> CHEBI_35696
ing13_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_007598 --> ing13_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_7H2O -.-> CHEBI_32312
ing14_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_007598 --> ing14_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_5H2O -.-> CHEBI_31440
ing15_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_007598 --> ing15_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing15_MnSO4_xH2O -.-> CHEBI_86360
ing16_NiSO4_6H2O["NiSO4・6H2O"]
CultureMech_007598 --> ing16_NiSO4_6H2O
CHEBI_53001["CHEBI:53001"]
ing16_NiSO4_6H2O -.-> CHEBI_53001
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
902.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 800.0, 1.0, 1.0, 100.0] |
| 2 | Agar (Purified) | 15 G_PER_L | Role: Solidifying component | |
| 3 | Yeast extract (BD-Difco) | 0.05 G_PER_L | Properties: Undefined component | |
| 4 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.46 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.147 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
55.6 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
39.6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
12.93 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2HPO4・12H2O
HO4P.2Na 141.958 g/mol
|
55.51 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.017 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.01 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.08 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.005 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.06 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiSO4・6H2O
Ni.O4S 154.756 g/mol
|
0.01 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
A--solution (see below)
Dose: 100 G_PER_L
B--solution (see below)
Dose: 100 G_PER_L
Trace element solution (see below)
Dose: 0.02 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1082_MM4Y_Medium (CultureMech:007598)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.879647Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1082 |
| 2026-01-28T19:48:33.971956Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:56.369938+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:27.927071Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:49.943491Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007598 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.298515+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM4Y Medium" to "mm4y_medium" for consistent matching |
| 2026-05-16T09:03:07.402373+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:30.251127+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:21.093038+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:46.857165+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:05.569562+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7c54b181135aa9310b0b7b655df09f4068ea63e73e699e84edc51c73c08d379a |