eggc_liquid_medium

CultureMech:007619 bacterial COMPLEX LIQUID
Original name: EGGC Liquid Medium

Overview

External ontology
TOGO:M1100 — EGGC Liquid Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1100 Original source: JCM - JCM_M1036 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1036

Composition

flowchart LR
    CultureMech_007619["eggc_liquid_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007619 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Sodium_acetate["Sodium acetate"]
    CultureMech_007619 --> ing1_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing1_Sodium_acetate -.-> CHEBI_32954
    ing2_CaCO3["CaCO3"]
    CultureMech_007619 --> ing2_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing2_CaCO3 -.-> CHEBI_3311
    ing3_Glucose["Glucose"]
    CultureMech_007619 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_007619 --> ing4_Casamino_acids__BD_Difco_
    ing5_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007619 --> ing5_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_7H2O -.-> CHEBI_31795
    ing6_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007619 --> ing6_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_2H2O -.-> CHEBI_86158
    ing7_KCl["KCl"]
    CultureMech_007619 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007619 --> ing8__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing8__NH4_2SO4 -.-> CHEBI_62946
    ing9_0_5_M_Na2HPO4__NaH2PO4_buffer__pH_7_3_["0.5 M Na2HPO4--NaH2PO4 buffer (pH 7.3)"]
    CultureMech_007619 --> ing9_0_5_M_Na2HPO4__NaH2PO4_buffer__pH_7_3_
    ing10_FeCl3_6H2O["FeCl3・6H2O"]
    CultureMech_007619 --> ing10_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing10_FeCl3_6H2O -.-> CHEBI_86254

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
302.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 1.0, 1.0, 300.0]
2 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.3 G_PER_L CHEBI:32954
sodium acetate
Role: Buffer; Properties: Organic compound, Simple component, Defined component
3 CaCO3
CO3.Ca 100.086 g/mol
0.02 G_PER_L CHEBI:3311
calcium carbonate
Role: Mineral source, Protective agent; Properties: Defined component, Inorganic compound, Simple component
4 Glucose
C6H12O6 180.156 g/mol
0.6 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Organic compound, Simple component, Defined component
5 Casamino acids (BD-Difco) 0.3 G_PER_L Properties: Undefined component
6 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
10 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
7 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
4 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8 KCl
Cl.K 74.551 g/mol
10 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
9 (NH4)2SO4
2H4N.O4S 132.141 g/mol
20 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
10 0.5 M Na2HPO4--NaH2PO4 buffer (pH 7.3) 2 G_PER_L Role: Buffer; Properties: Defined component, Solution, Inorganic compound, Complex component
11 FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
0.15 G_PER_L CHEBI:86254
iron trichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

Solution A (see below)

Dose: 5 G_PER_L

Solution B (see below)

Dose: 8 G_PER_L

Vitamins mix solution (see Medium [M290])

Dose: 5 G_PER_L

Cross-reference to Medium M290

FeCl3 solution (see below)

Dose: 1 G_PER_L

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:53.917998Z togo-import Imported from TOGO Medium Source: JCM, ID: M1100
2026-01-28T19:48:06.840686Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-20T07:49:55.000000Z organism-data-backfill Added curated organism data Added organism: EGGC Liquid, culture_type: isolate
2026-03-09T08:04:11.018155+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T03:08:37.667059Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:50.106802Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007619
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.468309+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "EGGC Liquid Medium" to "eggc_liquid_medium" for consistent matching
2026-05-16T09:03:07.569130+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:30.428982+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:21.210043+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:46.911249+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:05.741218+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 663b4a64878480c7d6d2dcb53a8cd5ee9f1a492f36141b52e70c121b62d8b49a