Overview
- External ontology
- TOGO:M1104 — Methanobacterium Medium (VII)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1104 Original source: JCM - JCM_M1039 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1039 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_007623["methanobacterium_medium_vii"]
ing0_Distilled_water["Distilled water"]
CultureMech_007623 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_007623 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_007623 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_007623 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_007623 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_007623 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_007623 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_Resazurin["Resazurin"]
CultureMech_007623 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_007623 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_NaHCO3["NaHCO3"]
CultureMech_007623 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Sodium_acetate["Sodium acetate"]
CultureMech_007623 --> ing10_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing10_Sodium_acetate -.-> CHEBI_32954
ing11_Sodium_formate["Sodium formate"]
CultureMech_007623 --> ing11_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing11_Sodium_formate -.-> CHEBI_62965
ing12_Calf_brains["Calf brains"]
CultureMech_007623 --> ing12_Calf_brains
ing13_Beef_heart["Beef heart"]
CultureMech_007623 --> ing13_Beef_heart
ing14_Proteose_peptone["Proteose peptone"]
CultureMech_007623 --> ing14_Proteose_peptone
ing15_Dextrose["Dextrose"]
CultureMech_007623 --> ing15_Dextrose
CHEBI_17634["CHEBI:17634"]
ing15_Dextrose -.-> CHEBI_17634
ing16_Sodium_chloride["Sodium chloride"]
CultureMech_007623 --> ing16_Sodium_chloride
ing16_Sodium_chloride -.-> CHEBI_26710
ing17_Disodium_phosphate["Disodium phosphate"]
CultureMech_007623 --> ing17_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing17_Disodium_phosphate -.-> CHEBI_34683
ing18_Proteose_peptone__BD_Difco_["Proteose peptone (BD-Difco)"]
CultureMech_007623 --> ing18_Proteose_peptone__BD_Difco_
ing19_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007623 --> ing19_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing19_Carbon_dioxide_gas -.-> CHEBI_16526
ing20_N2["N2"]
CultureMech_007623 --> ing20_N2
CHEBI_17997["CHEBI:17997"]
ing20_N2 -.-> CHEBI_17997
ing21_Hydrogen_gas["Hydrogen gas"]
CultureMech_007623 --> ing21_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing21_Hydrogen_gas -.-> CHEBI_18276
ing22_Biotin["Biotin"]
CultureMech_007623 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_007623 --> ing23_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p__Aminobenzoic_acid -.-> CHEBI_30753
ing24_Thiamine_HCl["Thiamine·HCl"]
CultureMech_007623 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Pyridoxine_HCl["Pyridoxine·HCl"]
CultureMech_007623 --> ing25_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_HCl -.-> CHEBI_30961
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_007623 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_007623 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_007623 --> ing28_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_DL_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 9 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 12 |
Sodium formate
CHO2.Na 68.007 g/mol
|
2 G_PER_L |
CHEBI:62965
sodium formate
|
Properties: Defined component, Inorganic compound, Simple component |
| 13 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 14 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 15 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 16 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 17 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 18 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 19 | Proteose peptone (BD-Difco) | 6 G_PER_L | Properties: Undefined component | |
| 20 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 21 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 22 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
80 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Thiamine·HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
200 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Pyridoxine·HCl
C8H11NO3.HCl 205.641 g/mol
|
300 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 | Vitamin B12 | 100 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
200 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
5% Na2S·9H2O solution
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
5% L-Cysteine·HCl·H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
Vitamin solution* (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1104_Methanobacterium_Medium_VII (CultureMech:007623)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.924616Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1104 |
| 2026-01-28T19:48:22.811469Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:37.971070+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:07.724977Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:50.140114Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007623 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.502141+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanobacterium Medium (VII)" to "methanobacterium_medium_vii" for consistent matching |
| 2026-05-16T09:03:07.609311+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.470498+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:29.911288+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:21.247200+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:46.921325+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:24.561416+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5c954d41a04f6e669815f435c1746970f682632b4a02ab7d92d5f5c5ea050b4a |