Overview
- External ontology
- TOGO:M1109 — ML-15 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1109 Original source: JCM - JCM_M1044 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1044
Composition
flowchart LR
CultureMech_007628["ml_15_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007628 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007628 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NaHCO3["NaHCO3"]
CultureMech_007628 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_007628 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Na2CO3["Na2CO3"]
CultureMech_007628 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_Na2B4O7_10H2O["Na2B4O7・10H2O"]
CultureMech_007628 --> ing5_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing5_Na2B4O7_10H2O -.-> CHEBI_131366
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
70 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
6.8 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
32 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
Na2B4O7・10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
3.8 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
10% Yeast extract solution
Dose: 20 G_PER_L
Role: Nutrient source; Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1109_ML-15_Medium (CultureMech:007628)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.935659Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1109 |
| 2026-01-28T19:48:28.757152Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:47.661823+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:50.181093Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007628 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.544375+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ML-15 Medium" to "ml_15_medium" for consistent matching |
| 2026-05-16T09:03:07.643081+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:30.504431+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.939026+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:05.789595+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3caadce36cc2eb2f67418fb1bb3e860ce5fa2d95ae326bd27d8f93e17d475658 |