fastidious_anaerobe_broth_medium

CultureMech:007630 bacterial COMPLEX LIQUID
Original name: Fastidious Anaerobe Broth Medium

Overview

External ontology
TOGO:M1110 — Fastidious Anaerobe Broth Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1110 Original source: JCM - JCM_M1045 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1045

Composition

flowchart LR
    CultureMech_007630["fastidious_anaerobe_broth_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007630 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007630 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NaHCO3["NaHCO3"]
    CultureMech_007630 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_007630 --> ing3_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing3_Sodium_pyruvate -.-> CHEBI_50144
    ing4_Hemin["Hemin"]
    CultureMech_007630 --> ing4_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing4_Hemin -.-> CHEBI_50385
    ing5_L__Arginine["L--Arginine"]
    CultureMech_007630 --> ing5_L__Arginine
    CHEBI_16467["CHEBI:16467"]
    ing5_L__Arginine -.-> CHEBI_16467
    ing6_Glucose["Glucose"]
    CultureMech_007630 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Soluble_starch["Soluble starch"]
    CultureMech_007630 --> ing7_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing7_Soluble_starch -.-> CHEBI_28017
    ing8_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_007630 --> ing8_Bacto_peptone__BD_Difco_
    ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007630 --> ing9_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing10_Sodium_pyrophosphate["Sodium pyrophosphate"]
    CultureMech_007630 --> ing10_Sodium_pyrophosphate
    CHEBI_71240["CHEBI:71240"]
    ing10_Sodium_pyrophosphate -.-> CHEBI_71240
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007630 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007630 --> ing12_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing12_Nitrogen_gas -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 NaHCO3
CHO3.Na 84.006 g/mol
0.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1 G_PER_L CHEBI:50144
sodium pyruvate
Role: Carbon source; Properties: Defined component, Organic compound, Simple component
5 Hemin
C34H32ClFeN4O4 651.952 g/mol
0.01 G_PER_L CHEBI:50385
hemin
Role: Growth factor; Properties: Simple component, Defined component, Organic compound
6 L--Arginine
C6H14N4O2 174.204 g/mol
1 G_PER_L CHEBI:16467
L-arginine
Role: Nitrogen source; Properties: Simple component, Defined component, Organic compound
7 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Simple component, Defined component, Organic compound
8 Soluble starch 1 G_PER_L CHEBI:28017
starch
Role: Carbon source; Properties: Undefined component
9 Bacto peptone (BD-Difco) 23 G_PER_L Properties: Undefined component
10 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
Properties: Defined component, Simple component, Organic compound
11 Sodium pyrophosphate
4Na.O7P2 265.901 g/mol
0.25 G_PER_L CHEBI:71240
sodium diphosphate
12 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
13 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: ガス

Solutions

250 mM Dithiothreitol solution

Dose: 4 G_PER_L

Properties: Defined component, Organic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:53.937657Z togo-import Imported from TOGO Medium Source: JCM, ID: M1110
2026-01-28T19:48:06.971434Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:11.252325+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:50.190679Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007630
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.554524+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Fastidious Anaerobe Broth Medium" to "fastidious_anaerobe_broth_medium" for consistent matching
2026-05-16T09:03:07.652040+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.513712+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:05.799178+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint bd70a82da4ded14ff0b5f8dae63549bb18d10d2b748cb2e13bdf3d4f26013f0a