acetomicrobium_faecale_medium

CultureMech:007645 bacterial COMPLEX LIQUID
Original name: Acetomicrobium Faecale Medium

Overview

External ontology
TOGO:M1124 — Acetomicrobium Faecale Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1124 Original source: JCM - JCM_M1057 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1057

Composition

flowchart LR
    CultureMech_007645["acetomicrobium_faecale_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007645 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007645 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_007645 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_007645 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007645 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_007645 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_K2HPO4["K2HPO4"]
    CultureMech_007645 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Resazurin["Resazurin"]
    CultureMech_007645 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007645 --> ing8__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing8__NH4_2SO4 -.-> CHEBI_62946
    ing9_Sodium_acetate_3H2O["Sodium acetate・3H2O"]
    CultureMech_007645 --> ing9_Sodium_acetate_3H2O
    CHEBI_32954["CHEBI:32954"]
    ing9_Sodium_acetate_3H2O -.-> CHEBI_32954
    ing10_Glucose["Glucose"]
    CultureMech_007645 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_007645 --> ing11_Trypticase_peptone__BD_BBL_
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007645 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007645 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997
    ing14_NaHCO3["NaHCO3"]
    CultureMech_007645 --> ing14_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing14_NaHCO3 -.-> CHEBI_32139
    ing15_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_007645 --> ing15_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing15_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing16_NiCl2_6H2O["NiCl2・6H2O"]
    CultureMech_007645 --> ing16_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_6H2O -.-> CHEBI_34887
    ing17_Na2SeO3_5H2O["Na2SeO3・5H2O"]
    CultureMech_007645 --> ing17_Na2SeO3_5H2O
    CHEBI_131361["CHEBI:131361"]
    ing17_Na2SeO3_5H2O -.-> CHEBI_131361

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 Yeast extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
0.5 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 KH2PO4
H2O4P.K 136.084 g/mol
0.225 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7 K2HPO4
HO4P.2K 174.174 g/mol
0.255 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
8 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
9 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.255 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
10 Sodium acetate・3H2O
C2H3O2.Na 82.034 g/mol
5 G_PER_L CHEBI:32954
sodium acetate
Role: Buffer; Properties: Organic compound, Defined component, Simple component
11 Glucose
C6H12O6 180.156 g/mol
4 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Organic compound, Defined component, Simple component
12 Trypticase peptone (BD-BBL) 2 G_PER_L Properties: Undefined component
13 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
14 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: ガス
15 NaHCO3
CHO3.Na 84.006 g/mol
6 G_PER_L CHEBI:32139
sodium hydrogencarbonate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
16 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
Properties: Defined component, Simple component, Organic compound
17 NiCl2・6H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
0.3 G_PER_L CHEBI:131361
disodium selenite pentahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Trace minerals solution (see below)

Dose: 10 G_PER_L

Trace minerals (see Medium [M142])

Dose: 1 G_PER_L

Cross-reference to Medium M142

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:53.966121Z togo-import Imported from TOGO Medium Source: JCM, ID: M1124
2026-01-28T19:48:16.839771Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:27.819838+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:08:56.575503Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:50.309161Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007645
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:08.676792+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Acetomicrobium Faecale Medium" to "acetomicrobium_faecale_medium" for consistent matching
2026-05-16T09:03:07.730221+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:30.605697+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:21.346635+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:46.968994+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:05.889534+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 76657360d6359908211afe4e70d8365399674854cee6f144ec650844e65f16fd