Overview
- External ontology
- TOGO:M1136 — Freshwater Iron-Oxidizing Bacteria Medium-II
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1136 Original source: JCM - JCM_M1067 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1067
Composition
flowchart LR
CultureMech_007658["freshwater_iron_oxidizing_bacteria_medium_ii"]
ing0_Bottom_layer["Bottom layer"]
CultureMech_007658 --> ing0_Bottom_layer
ing1_Top_layer["Top layer"]
CultureMech_007658 --> ing1_Top_layer
ing2_Agar__Noble__BD_Difco_["Agar, Noble (BD-Difco)"]
CultureMech_007658 --> ing2_Agar__Noble__BD_Difco_
ing3_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007658 --> ing3_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing3_Carbon_dioxide_gas -.-> CHEBI_16526
ing4_Nitrogen_gas["Nitrogen gas"]
CultureMech_007658 --> ing4_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing4_Nitrogen_gas -.-> CHEBI_17997
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bottom layer | 100 G_PER_L | ||
| 2 | Top layer | 1010 G_PER_L | ||
| 3 | Agar, Noble (BD-Difco) | 1.5 G_PER_L | Role: Solidifying component; Properties: Defined component | |
| 4 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 5 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
FeS solution (see Medium [M641])
Dose: 50 G_PER_L
Cross-reference to Medium M641
Modified Wolfe's solution (see Medium [M641])
Dose: 50 G_PER_L
Cross-reference to Medium M641
Modified Wolfe's solution (see Medium [M641])
Dose: 1 G_PER_L
Cross-reference to Medium M641
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
8% NaHCO3 solution
Dose: 5.25 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1136_Freshwater_Iron-Oxidizing_Bacteria_Medium-II (CultureMech:007658)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.987720Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1136 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:05:04.641732+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:19:50.397246Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007658 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T19:46:08.769351+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Freshwater Iron-Oxidizing Bacteria Medium-II" to "freshwater_iron_oxidizing_bacteria_medium_ii" for consistent matching |
| 2026-05-16T09:03:07.818418+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-08-06T03:05:05.972580+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 16b06379c7b37c7030f0cb4f409571055cb09801848871c78659f27054d029c2 |