Overview
- External ontology
- TOGO:M1158 — 0.3 x Hyphomicrobium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1158 Original source: JCM - JCM_M1088 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1088
Composition
flowchart LR
CultureMech_007681["0_3_x_hyphomicrobium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007681 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007681 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_007681 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_007681 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Methanol["Methanol"]
CultureMech_007681 --> ing4_Methanol
CHEBI_17790["CHEBI:17790"]
ing4_Methanol -.-> CHEBI_17790
ing5_NaH2PO4["NaH2PO4"]
CultureMech_007681 --> ing5_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing5_NaH2PO4 -.-> CHEBI_37585
ing6_Agar["Agar"]
CultureMech_007681 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Trace_vitamins__see_below_["Trace vitamins (see below)"]
CultureMech_007681 --> ing7_Trace_vitamins__see_below_
ing8_Thiamine_HCl["Thiamine・HCl"]
CultureMech_007681 --> ing8_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing8_Thiamine_HCl -.-> CHEBI_49105
ing9_Folic_acid["Folic acid"]
CultureMech_007681 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_007681 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
ing11_Riboflavin["Riboflavin"]
CultureMech_007681 --> ing11_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing11_Riboflavin -.-> CHEBI_17015
ing12_Niacin["Niacin"]
CultureMech_007681 --> ing12_Niacin
CHEBI_15940["CHEBI:15940"]
ing12_Niacin -.-> CHEBI_15940
ing13_Ascorbic_acid["Ascorbic acid"]
CultureMech_007681 --> ing13_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing13_Ascorbic_acid -.-> CHEBI_22652
ing14_Pantothenic_acid["Pantothenic acid"]
CultureMech_007681 --> ing14_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing14_Pantothenic_acid -.-> CHEBI_7916
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
501.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 500.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.07 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.85 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Methanol
CH4O 32.042 g/mol
|
2 G_PER_L |
CHEBI:17790
methanol
|
Role: Solvating media; Properties: Organic compound, Defined component, Simple component |
| 6 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
0.75 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Trace vitamins (see below) | 2 G_PER_L | ||
| 9 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
25 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
12.5 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 11 | Vitamin B12 | 125 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
25 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Niacin
C6H5NO2 123.111 g/mol
|
25 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 | Ascorbic acid | 40 G_PER_L |
CHEBI:22652
ascorbic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
25 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Visniac trace elements (see Medium [M925])
Dose: 1 G_PER_L
Cross-reference to Medium M925
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1158_0.3_x_Hyphomicrobium_Medium (CultureMech:007681)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.028245Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1158 |
| 2026-01-28T19:48:39.798102Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:06.981034+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:38.699374Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:50.577326Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007681 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:08.975076+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "0.3 x Hyphomicrobium Medium" to "0_3_x_hyphomicrobium_medium" for consistent matching |
| 2026-05-16T09:03:08.008785+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:30.882131+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:21.553315+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.065074+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:06.183745+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ea4b60834e68d7f1ef89e03b912be6d026fbf80e90090124fee20d9e1b9143fd |