Overview
- External ontology
- TOGO:M1163 — MJ Basal Medium With Thiosulfate/2
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1163 Original source: JCM - JCM_M1093 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1093
Composition
flowchart LR
CultureMech_007687["mj_basal_medium_with_thiosulfate_2"]
ing0_Distilled_water["Distilled water"]
CultureMech_007687 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NH4Cl["NH4Cl"]
CultureMech_007687 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KNO3["KNO3"]
CultureMech_007687 --> ing2_KNO3
CHEBI_63043["CHEBI:63043"]
ing2_KNO3 -.-> CHEBI_63043
ing3_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007687 --> ing3_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing3_Carbon_dioxide_gas -.-> CHEBI_16526
ing4_Nitrogen_gas["Nitrogen gas"]
CultureMech_007687 --> ing4_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing4_Nitrogen_gas -.-> CHEBI_17997
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
KNO3
K.NO3 101.102 g/mol
|
0.25 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 5 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
MJ(-N) synthetic seawater (see Medium [M260])
Dose: 1 G_PER_L
Cross-reference to Medium M260
8% NaHCO3 solution
Dose: 10 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
10% Na2S2O3・5H2O solution
Dose: 15 G_PER_L
Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1163_MJ_Basal_Medium_With_Thiosulfate_2 (CultureMech:007687)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.037089Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1163 |
| 2026-01-28T19:48:33.653446Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:55.791613+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:19:50.614560Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007687 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.014716+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ Basal Medium With Thiosulfate/2" to "mj_basal_medium_with_thiosulfate_2" for consistent matching |
| 2026-05-16T09:03:08.050546+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:30.925252+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.077499+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:06.226221+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bb79c1bb9986cb14354d294c76ece99cc013fcd52c685202ed8cc7835eb43cdd |