Overview
- External ontology
- TOGO:M1169 — PYGA Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1169 Original source: JCM - JCM_M1096 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1096
Composition
flowchart LR
CultureMech_007693["pyga_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007693 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007693 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_007693 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Glucose["Glucose"]
CultureMech_007693 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Agar["Agar"]
CultureMech_007693 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Peptone["Peptone"]
CultureMech_007693 --> ing5_Peptone
ing6_NaHCO3["NaHCO3"]
CultureMech_007693 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Na2CO3["Na2CO3"]
CultureMech_007693 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 900.0, 100.0] |
| 2 | Yeast extract | 3 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 | Peptone | 8 G_PER_L | ||
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.9 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
7 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Solution 1
Dose: 900 G_PER_L
See mediadive_6108_Solution_1.yaml for composition
Solution 2
Dose: 100 G_PER_L
See mediadive_6109_Solution_2.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1169_PYGA_Medium (CultureMech:007693)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.048646Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1169 |
| 2026-01-28T19:48:29.011302Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:48.102353+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:19.015186Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:50.665933Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007693 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.067708+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYGA Medium" to "pyga_medium" for consistent matching |
| 2026-05-16T09:03:08.104005+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:30.979675+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.098779+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:06.272529+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 893d72d8370684d76de8a75294a8c3dc9aedd81807203479a5adc672d0927c7f |