azc_1502_medium

CultureMech:007724 bacterial COMPLEX LIQUID
Original name: AZC 1502 Medium

Overview

External ontology
TOGO:M1199 — AZC 1502 Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1199 Original source: JCM - JCM_M1121 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1121

Composition

flowchart LR
    CultureMech_007724["azc_1502_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007724 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007724 --> ing1_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_2H2O -.-> CHEBI_86158
    ing2_KH2PO4["KH2PO4"]
    CultureMech_007724 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007724 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007724 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_007724 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Na2S2O3_5H2O["Na2S2O3・5H2O"]
    CultureMech_007724 --> ing6_Na2S2O3_5H2O
    CHEBI_32150["CHEBI:32150"]
    ing6_Na2S2O3_5H2O -.-> CHEBI_32150
    ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007724 --> ing7_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing7_Carbon_dioxide_gas -.-> CHEBI_16526
    ing8_N2["N2"]
    CultureMech_007724 --> ing8_N2
    CHEBI_17997["CHEBI:17997"]
    ing8_N2 -.-> CHEBI_17997
    ing9_Hydrogen_gas["Hydrogen gas"]
    CultureMech_007724 --> ing9_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing9_Hydrogen_gas -.-> CHEBI_18276
    ing10_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_007724 --> ing10_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_7H2O -.-> CHEBI_75836
    ing11_Na2SeO3_5H2O["Na2SeO3・5H2O"]
    CultureMech_007724 --> ing11_Na2SeO3_5H2O
    CHEBI_131361["CHEBI:131361"]
    ing11_Na2SeO3_5H2O -.-> CHEBI_131361
    ing12_Na2WO4_2H2O["Na2WO4・2H2O"]
    CultureMech_007724 --> ing12_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_2H2O -.-> CHEBI_63939
    ing13_NiSO4_7H2O["NiSO4・7H2O"]
    CultureMech_007724 --> ing13_NiSO4_7H2O
    CHEBI_53001["CHEBI:53001"]
    ing13_NiSO4_7H2O -.-> CHEBI_53001

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
2.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 1.0]
2 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 NH4Cl
Cl.H4N 53.492 g/mol
0.1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
5 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 KCl
Cl.K 74.551 g/mol
0.05 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
3.2 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
 Properties: Defined component, Inorganic compound, Simple component
8 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
9 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
10 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
11 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
1.42 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
12 Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
3 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
13 Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
38 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
14 NiSO4・7H2O
Ni.O4S 154.756 g/mol
1.6 G_PER_L CHEBI:53001
nickel sulfate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Fe--Ni--WO--Se solution (see below)

Dose: 1 G_PER_L

1% Yeast extract solution

Dose: 10 G_PER_L

Role: Nutrient source; Properties: Undefined component

1 M Sodium acetate solution

Dose: 10 G_PER_L

Role: Buffer; Properties: Defined component, Solution, Organic compound

0.1% Sodium dithionite solution

Dose: 20 G_PER_L

Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.108833Z togo-import Imported from TOGO Medium Source: JCM, ID: M1199
2026-01-28T19:48:29.275804Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:48.615567+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T03:09:19.726170Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:50.926866Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007724
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.334942+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AZC 1502 Medium" to "azc_1502_medium" for consistent matching
2026-05-16T09:03:08.342843+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.231575+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.181652+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:06.600104+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ecdf4355f2079ac4fa7b5ddfefc70784683c5da9331649f9faaaff32e05f6d21