Overview
- External ontology
- TOGO:M1205 — Sulfate-Free Metako Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1205 Original source: JCM - JCM_M1126 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1126
Composition
flowchart LR
CultureMech_007732["sulfate_free_metako_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007732 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007732 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007732 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_007732 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_007732 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_007732 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007732 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_007732 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_007732 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_007732 --> ing9_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing9_SrCl2_6H2O -.-> CHEBI_36385
ing10_NaF["NaF"]
CultureMech_007732 --> ing10_NaF
CHEBI_28741["CHEBI:28741"]
ing10_NaF -.-> CHEBI_28741
ing11_KBr["KBr"]
CultureMech_007732 --> ing11_KBr
CHEBI_32030["CHEBI:32030"]
ing11_KBr -.-> CHEBI_32030
ing12_LiCl["LiCl"]
CultureMech_007732 --> ing12_LiCl
CHEBI_48607["CHEBI:48607"]
ing12_LiCl -.-> CHEBI_48607
ing13_Na2B4O7_10H2O["Na2B4O7・10H2O"]
CultureMech_007732 --> ing13_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing13_Na2B4O7_10H2O -.-> CHEBI_131366
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007732 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_Nitrogen_gas["Nitrogen gas"]
CultureMech_007732 --> ing15_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing15_Nitrogen_gas -.-> CHEBI_17997
ing16_N2["N2"]
CultureMech_007732 --> ing16_N2
ing16_N2 -.-> CHEBI_17997
ing17_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007732 --> ing17_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_2H2O -.-> CHEBI_75213
ing18_H3BO3["H3BO3"]
CultureMech_007732 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007732 --> ing19_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing19_MnCl2_4H2O -.-> CHEBI_86368
ing20_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_007732 --> ing20_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing20_CoCl2_6H2O -.-> CHEBI_35696
ing21_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_007732 --> ing21_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_6H2O -.-> CHEBI_34887
ing22_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_007732 --> ing22_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2_2H2O -.-> CHEBI_86318
ing23_ZnCl2["ZnCl2"]
CultureMech_007732 --> ing23_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing23_ZnCl2 -.-> CHEBI_49976
ing24_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_007732 --> ing24_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing24_FeCl3_6H2O -.-> CHEBI_86254
ing25_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_007732 --> ing25_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing25_Nitrilotriacetic_acid -.-> CHEBI_44557
ing26_NaBr["NaBr"]
CultureMech_007732 --> ing26_NaBr
CHEBI_63004["CHEBI:63004"]
ing26_NaBr -.-> CHEBI_63004
ing27_KI["KI"]
CultureMech_007732 --> ing27_KI
CHEBI_8346["CHEBI:8346"]
ing27_KI -.-> CHEBI_8346
ing28_AlCl3["AlCl3"]
CultureMech_007732 --> ing28_AlCl3
CHEBI_30114["CHEBI:30114"]
ing28_AlCl3 -.-> CHEBI_30114
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
20.75 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.36 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.54 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
9.47 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.6 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.69 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
12.01 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 12.0, 0.01] |
| 11 |
NaF
F.Na 41.988 g/mol
|
3 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
KBr
Br.K 119.002 g/mol
|
85 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
LiCl
Cl.Li 42.394 g/mol
|
1 G_PER_L |
CHEBI:48607
lithium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2B4O7・10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
34 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 16 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 18 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.61 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.42 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.007 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.09 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.5 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.15 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 27 |
NaBr
Br.Na 102.894 g/mol
|
0.01 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
KI
I.K 166.002 g/mol
|
0.07 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
AlCl3
AlCl3 133.341 g/mol
|
0.006 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution (see below)
Dose: 1 G_PER_L
Selenite--tungstate solution (see Medium [M431])
Dose: 0.4 G_PER_L
Cross-reference to Medium M431
Trace vitamins solution (see Medium [M701])
Dose: 2 G_PER_L
Cross-reference to Medium M701
1 M Trimethylamine solution
Dose: 10 G_PER_L
Role: Nutrient source
5% Na2S・9H2O solution
Dose: 2.5 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
5% L--Cysteine・HCl・H2O solution
Dose: 2.5 G_PER_L
Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1205_Sulfate-Free_Metako_Medium (CultureMech:007732)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.126227Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1205 |
| 2026-01-28T19:48:16.680840Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:27.554503+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:08:56.293717Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:51.007148Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007732 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.416137+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sulfate-Free Metako Medium" to "sulfate_free_metako_medium" for consistent matching |
| 2026-05-16T09:03:08.421793+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.312021+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.210558+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:06.689770+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f69e2e4af5359991c5a3d70d0a9c5d604553e3245af7ee62fa844a9646e74a52 |