Overview
- External ontology
- TOGO:M1217 — Roseibaca Calidilacus Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1217 Original source: JCM - JCM_M1137 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1137
Composition
flowchart LR
CultureMech_007745["roseibaca_calidilacus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007745 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_007745 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaHCO3["NaHCO3"]
CultureMech_007745 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Na2CO3["Na2CO3"]
CultureMech_007745 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Glycerol["Glycerol"]
CultureMech_007745 --> ing4_Glycerol
CHEBI_17754["CHEBI:17754"]
ing4_Glycerol -.-> CHEBI_17754
ing5_Ammonium_Fe_III__citrate["Ammonium Fe(III) citrate"]
CultureMech_007745 --> ing5_Ammonium_Fe_III__citrate
ing6_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007745 --> ing6_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_7H2O -.-> CHEBI_31795
ing7_KCl["KCl"]
CultureMech_007745 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaSO4["NaSO4"]
CultureMech_007745 --> ing8_NaSO4
CHEBI_32149["CHEBI:32149"]
ing8_NaSO4 -.-> CHEBI_32149
ing9_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007745 --> ing9_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_2H2O -.-> CHEBI_86158
ing10_NaNO3["NaNO3"]
CultureMech_007745 --> ing10_NaNO3
CHEBI_63005["CHEBI:63005"]
ing10_NaNO3 -.-> CHEBI_63005
ing11_EDTA_2Na["EDTA・2Na"]
CultureMech_007745 --> ing11_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing11_EDTA_2Na -.-> CHEBI_64734
ing12_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007745 --> ing12_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_007745 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007745 --> ing14_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_4H2O -.-> CHEBI_86368
ing15_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_007745 --> ing15_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_7H2O -.-> CHEBI_32312
ing16_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_007745 --> ing16_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_5H2O -.-> CHEBI_31440
ing17_Ca_NO3_2_6H2O["Ca(NO3)2・6H2O"]
CultureMech_007745 --> ing17_Ca_NO3_2_6H2O
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1672.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 770.0, 1.0, 900.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.03 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.84 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.02 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Glycerol
C3H8O3 92.094 g/mol
|
0.46 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 | Ammonium Fe(III) citrate | 1.42 G_PER_L | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component | |
| 7 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
500.49 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 493.0, 7.49] |
| 8 |
KCl
Cl.K 74.551 g/mol
|
7.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaSO4
2Na.O4S 142.043 g/mol
|
57 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaNO3
NO3.Na 84.994 g/mol
|
149.58 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
0.1 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 13 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
390 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
79 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 | Ca(NO3)2・6H2O | 49.4 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component, Mineral source |
Solutions
1 M TES solution (pH 8.0)
Dose: 10 G_PER_L
Role: Buffer; Properties: Defined component, Organic compound, Simple component
5 x Salts solution (see below)
Dose: 200 G_PER_L
100 x BG--FPC solution (see below)
Dose: 10 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Trace element solution (see below)
Dose: 100 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1217_Roseibaca_Calidilacus_Medium (CultureMech:007745)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.151564Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1217 |
| 2026-01-28T19:48:34.956259Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:58.036418+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:29.668582Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:51.121321Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007745 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.535998+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Roseibaca Calidilacus Medium" to "roseibaca_calidilacus_medium" for consistent matching |
| 2026-05-16T09:03:08.546920+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:31.442209+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:30.074797+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:21.978483+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.261950+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:06.832708+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint abe7e59456d5a4be5be168007ecaf3263e0c6d836474bab7b099f38b15a30986 |